Toxicity of Mitoxantrone-loaded Superparamagnetic Iron Oxide Nanoparticles in a HT-29 Tumour Spheroid Model

Journal article


Publication Details

Author(s): Hornung A, Poettler M, Friedrich RP, Weigel B, Dürr S, Zaloga J, Cicha I, Alexiou C, Janko C
Journal: Anticancer Research
Publication year: 2016
Volume: 36
Journal issue: 6
Pages range: 3093-101
ISSN: 0250-7005


Abstract


Cancer research is commonly carried out in two-dimensional (2D) cell cultures, which poorly reflect in vivo settings where the growing tumours are exposed to mechanical forces and biochemical gradients. In this study we established a HF-29 colon carcinoma tumor spheroid model to investigate the effect of free mitoxantrone (MTO) and its nanoparticle-bound form (SPION(MTO)) under 3D cell culture conditions.Tumour spheroids were generated by seeding HT-29 colon carcinoma cells on agarose-coated cell culture wells. Growth of the spheroids was monitored daily by transmission microscopy upon treatment with free MTO, SPION(MTO) or unloaded SPION.Unloaded SPION did not affect the spheroid size compared to untreated controls, while both free MTO and SPION(MTO) inhibited growth of the spheroids in a dose- and time-dependent manner. In comparison to free MTO, the effect of SPION(MTO) on spheroid growth was slightly delayed. Further analyses are necessary to investigate if MTO infiltrates spheroids in its nanoparticle-bound form or whether it is released from SPION before infiltration.



FAU Authors / FAU Editors

Alexiou, Christoph Prof. Dr.
Professur für Nanomedizin
Janko, Christina Dr. rer. nat.
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie
Zaloga, Jan Dr. rer. nat.
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie


Additional Organisation
Exzellenz-Cluster Engineering of Advanced Materials


Research Fields

Nanosafety
Exzellenz-Cluster Engineering of Advanced Materials


How to cite

APA:
Hornung, A., Poettler, M., Friedrich, R.P., Weigel, B., Dürr, S., Zaloga, J.,... Janko, C. (2016). Toxicity of Mitoxantrone-loaded Superparamagnetic Iron Oxide Nanoparticles in a HT-29 Tumour Spheroid Model. Anticancer Research, 36(6), 3093-101.

MLA:
Hornung, Annkathrin, et al. "Toxicity of Mitoxantrone-loaded Superparamagnetic Iron Oxide Nanoparticles in a HT-29 Tumour Spheroid Model." Anticancer Research 36.6 (2016): 3093-101.

BibTeX: 

Last updated on 2018-10-10 at 08:27