Chlorella thermophila (Trebouxiophyceae, Chlorophyta), a novel thermo-tolerant Chlorella species isolated from an occupied rooftop incubator

Ma S, Han B, Huß V, Hu X, Sun X, Zhang J (2015)


Publication Language: English

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2015

Journal

Publisher: Kluwer Academic Publishers

Book Volume: 760

Pages Range: 81-89

Journal Issue: 1

DOI: 10.1007/s10750-015-2304-3

Abstract

Open rooftop incubators may provide a cost-effective way for producing microalgal biomass for biodiesel production without requiring large land areas. While cultivating a Chlorella vulgaris strain with high oil content in a rooftop incubator, we identified an invading thermo-resistant green alga, which replaced the original culture. The isolated strain HTA1-65 had the typical Chlorella morphology with spherical cells and a cup-shaped parietal chloroplast. The protoplast contained a single pyrenoid traversed by a double-layered thylakoid and enveloped by a single starch sheath. The oil content of this strain was only 21.5 %, one third of the original strain. This strain grew at 42 °C, with an optimum of 33 °C. Heat treatment of 45 °C for 3 h per day did not decrease its growth rate, suggesting that it has a high potential in biotechnological applications. Phylogenetic analyses revealed that HTA1-65 belongs to the genus Chlorella, but a specific relationship to any species was not indicated by nuclear and chloroplast small subunit rRNA genes. However, based on at least one compensating base change (CBC) and a few hemi-CBCs in the ITS2 secondary structure of closely related species, we describe strain HTA1-65 as a novel, thermo-tolerant Chlorella species, C. thermophila.

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APA:

Ma, S., Han, B., Huß, V., Hu, X., Sun, X., & Zhang, J. (2015). Chlorella thermophila (Trebouxiophyceae, Chlorophyta), a novel thermo-tolerant Chlorella species isolated from an occupied rooftop incubator. Hydrobiologia, 760(1), 81-89. https://dx.doi.org/10.1007/s10750-015-2304-3

MLA:

Ma, Shuai, et al. "Chlorella thermophila (Trebouxiophyceae, Chlorophyta), a novel thermo-tolerant Chlorella species isolated from an occupied rooftop incubator." Hydrobiologia 760.1 (2015): 81-89.

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