A 2ND L-TYPE ISOZYME OF POTATO GLUCAN PHOSPHORYLASE - CLONING, ANTISENSE INHIBITION AND EXPRESSION ANALYSIS

Journal article


Publication Details

Author(s): Sonnewald U
Journal: Plant Molecular Biology
Publisher: SPRINGER
Publication year: 1995
Volume: 27
Journal issue: 3
Pages range: 567-576
ISSN: 0167-4412


Abstract


Northern analysis revealed that both forms are differentially expressed. The steady-state mRNA levels of the tuber L-type isozyme accumulates strongly in potato tubers and only weakly in leaf tissues, whereas the mRNA of the leaf L-type isozyme accumulates in both tissues to the same extent. Constitutive expression of an antisense RNA specific for the leaf L-type gene resulted in a strong reduction of starch phosphorylase L-type activity in leaf tissue, but had only sparse effects in potato tuber tissues. Determination of the leaf starch content revealed that antisense repression of the starch phosphorylase activity has no significant influence on starch accumulation in leaves of transgenic potato plants. This result indicated that different L-type genes are responsible for the starch phosphorylase activity in different tissues, but the function of the different enzymes remains unclear.



FAU Authors / FAU Editors

Sonnewald, Uwe Prof. Dr.
Lehrstuhl für Biochemie

Last updated on 2018-08-08 at 17:39