PEDF Is Associated with the Termination of Chondrocyte Phenotype and Catabolism of Cartilage Tissue

Beitrag in einer Fachzeitschrift


Details zur Publikation

Autorinnen und Autoren: Klinger P, Lukassen S, Ferrazzi F, Ekici AB, Hotfiel T, Swoboda B, Aigner T, Gelse K
Zeitschrift: BioMed Research International
Jahr der Veröffentlichung: 2017
Band: 2017
Seitenbereich: 7183516
ISSN: 2314-6133


Abstract

Objective. To investigate the expression and target genes of pigment epithelium-derived factor (PEDF) in cartilage and chondrocytes, respectively. Methods. We analyzed the expression pattern of PEDF in different human cartilaginous tissues including articular cartilage, osteophytic cartilage, and fetal epiphyseal and growth plate cartilage, by immunohistochemistry and quantitative real-time (qRT) PCR. Transcriptome analysis after stimulation of human articular chondrocytes with rhPEDF was performed by RNA sequencing (RNA-Seq) and confirmed by qRT-PCR. Results. Immunohistochemically, PEDF could be detected in transient cartilaginous tissue that is prone to undergo endochondral ossification, including epiphyseal cartilage, growth plate cartilage, and osteophytic cartilage. In contrast, PEDF was hardly detected in healthy articular cartilage and in the superficial zone of epiphyses, regions that are characterized by a permanent stable chondrocyte phenotype. RNA-Seq analysis and qRT-PCR demonstrated that rhPEDF significantly induced the expression of a number of matrix-degrading factors including SAA1, MMP1, MMP3, and MMP13. Simultaneously, a number of cartilage-specific genes including COL2A1, COL9A2, COMP, and LECT were among the most significantly downregulated genes. Conclusions. PEDF represents a marker for transient cartilage during all neonatal and postnatal developmental stages and promotes the termination of cartilage tissue by upregulation of matrix-degrading factors and downregulation of cartilage-specific genes. These data provide the basis for novel strategies to stabilize the phenotype of articular cartilage and prevent its degradation.


FAU-Autorinnen und Autoren / FAU-Herausgeberinnen und Herausgeber

Ekici, Arif Bülent Dr. rer. nat.
Humangenetisches Institut
Ferrazzi, Fulvia Dr.
Humangenetisches Institut
Hotfiel, Thilo
Klinger, Patricia Dr. rer. nat.
Unfallchirurgische Abteilung in der Chirurgischen Klinik
Lukassen, Sören
Professur für Humangenetik
Swoboda, Bernd Prof. Dr.
Professur für Orthopädische Rheumatologie


Einrichtungen weiterer Autorinnen und Autoren

Klinikum Coburg


Zitierweisen

APA:
Klinger, P., Lukassen, S., Ferrazzi, F., Ekici, A.B., Hotfiel, T., Swoboda, B.,... Gelse, K. (2017). PEDF Is Associated with the Termination of Chondrocyte Phenotype and Catabolism of Cartilage Tissue. BioMed Research International, 2017, 7183516. https://dx.doi.org/10.1155/2017/7183516

MLA:
Klinger, Patricia, et al. "PEDF Is Associated with the Termination of Chondrocyte Phenotype and Catabolism of Cartilage Tissue." BioMed Research International 2017 (2017): 7183516.

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Zuletzt aktualisiert 2019-02-04 um 08:48