Direct uptake of Antagomirs and efficient knockdown of miRNA in primary B and T lymphocytes
Haftmann C, Riedel R, Porstner M, Wittmann J, Chang HD, Radbruch A, Mashreghi MF (2015)
Publication Type: Journal article
Publication year: 2015
Journal
Book Volume: 426
Pages Range: 128-33
DOI: 10.1016/j.jim.2015.07.006
Abstract
Depending on their physiological expression level, microRNAs (miRNA) address different target genes, thus have different biological functions. To identify these, the physiological expression has to be blocked. Here, we describe the use of inhibitory cholesterol-modified oligonucleotides (Antagomirs) to inhibit miRNAs selectively in primary human and murine T and B lymphocytes. Due to their lipophilic cholesterol tag Antagomirs enter primary lymphocytes efficiently and quantitatively. We show here that at concentrations of 0.125 to 1?M, Antagomirs selectively inhibit expression of their target miRNA up to 99.5% without affecting cell viability.
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Germany (DE)How to cite
APA:
Haftmann, C., Riedel, R., Porstner, M., Wittmann, J., Chang, H.-D., Radbruch, A., & Mashreghi, M.-F. (2015). Direct uptake of Antagomirs and efficient knockdown of miRNA in primary B and T lymphocytes. Journal of Immunological Methods, 426, 128-33. https://doi.org/10.1016/j.jim.2015.07.006
MLA:
Haftmann, Claudia, et al. "Direct uptake of Antagomirs and efficient knockdown of miRNA in primary B and T lymphocytes." Journal of Immunological Methods 426 (2015): 128-33.
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