Nanometric thermal fluctuations of weakly confined biomembranes measured with microsecond time-resolution

Beitrag in einer Fachzeitschrift
(Originalarbeit)


Details zur Publikation

Autor(en): Monzel C, Schmidt D, Seifert U, Smith AS, Merkel R, Sengupta K
Zeitschrift: Soft Matter
Verlag: ROYAL SOC CHEMISTRY
Jahr der Veröffentlichung: 2016
Band: 12
Heftnummer: 21
Seitenbereich: 4755-4768
ISSN: 1744-683X
Sprache: Englisch


Abstract


We probe the bending fluctuations of bio-membranes using highly deflated giant unilamellar vesicles (GUVs) bound to a substrate by a weak potential arising from generic interactions. The substrate is either homogeneous, with GUVs bound only by the weak potential, or is chemically functionalized with a micro-pattern of very strong specific binders. In both cases, the weakly adhered membrane is seen to be confined at a well-defined distance above the surface while it continues to fluctuate strongly. We quantify the fluctuations of the weakly confined membrane at the substrate proximal surface as well as of the free membrane at the distal surface of the same GUV. This strategy enables us to probe in detail the damping of fluctuations in the presence of the substrate, and to independently measure the membrane tension and the strength of the generic interaction potential. Measurements were done using two complementary techniques - dynamic optical displacement spectroscopy (DODS, resolution: 20 nm, 10 ms), and dual wavelength reflection interference contrast microscopy (DW-RICM, resolution: 4 nm, 50 ms). After accounting for the spatio-temporal resolution of the techniques, an excellent agreement between the two measurements was obtained. For both weakly confined systems we explore in detail the link between fluctuations on the one hand and membrane tension and the interaction potential on the other hand.



FAU-Autoren / FAU-Herausgeber

Smith, Ana-Suncana Prof. Dr.
Professur für Theoretische Physik


Zusätzliche Organisationseinheit(en)
Exzellenz-Cluster Engineering of Advanced Materials


Autor(en) der externen Einrichtung(en)
Aix-Marseille University / Aix-Marseille Université
Forschungszentrum Jülich GmbH (FZJ)
Universität Stuttgart


Zitierweisen

APA:
Monzel, C., Schmidt, D., Seifert, U., Smith, A.-S., Merkel, R., & Sengupta, K. (2016). Nanometric thermal fluctuations of weakly confined biomembranes measured with microsecond time-resolution. Soft Matter, 12(21), 4755-4768. https://dx.doi.org/10.1039/c6sm00412a

MLA:
Monzel, Cornelia, et al. "Nanometric thermal fluctuations of weakly confined biomembranes measured with microsecond time-resolution." Soft Matter 12.21 (2016): 4755-4768.

BibTeX: 

Zuletzt aktualisiert 2018-08-08 um 16:53