Zingler C, Carl HD, Swoboda B, Krinner S, Hennig F, Gelse K (2016)
Publication Type: Journal article
Publication year: 2016
Book Volume: 24
Pages Range: 124-8
Journal Issue: 1
DOI: 10.1016/j.joca.2015.07.014
Cellular outgrowth from articular cartilage tissue has been described in a number of recent experimental studies. The aim of this study was to investigate the occurrence of cellular outgrowth from articular cartilage explants isolated from adult human donors.Macroscopically intact articular cartilage specimens were isolated from adult human donors and cultured either in their native status, or in a cleansed status achieved by forced washing to minimize attaching cells. Additionally, the effect of chemotactic stimuli including cell lysate, High-Mobility-Group-Protein B1 (HMGB-1), Trefoil-factor 3 (TFF3), bone morphogenetic protein-2 (BMP-2), transforming growth factor-ß1 (TGF-ß1), or three-dimensional fibrin or collagen matrices were investigated. Co-cultures with synovial membrane served as a positive control for a source of migratory cells. The occurrence of cellular outgrowth was analyzed by histological examination after a culture period of 4 weeks.Spontaneous cellular outgrowth from cleansed cartilage specimens was not observed at a relevant level and could not significantly be induced by chemotactic stimuli or three-dimensional matrices either. A forming cartilage-adjoining cell layer was only apparent in the case of native cartilage explants with cellular remnants from surgical isolation or in co-culture experiments with synovial membrane.The relevance of cellular outgrowth from cartilage tissue is largely absent in the case of adult human articular cartilage samples. A cartilage-adjoining cell layer forming around the explants may instead originate from still attaching cells that remained from surgical isolation.
APA:
Zingler, C., Carl, H.-D., Swoboda, B., Krinner, S., Hennig, F., & Gelse, K. (2016). Limited evidence of chondrocyte outgrowth from adult human articular cartilage. Osteoarthritis and Cartilage, 24(1), 124-8. https://doi.org/10.1016/j.joca.2015.07.014
MLA:
Zingler, Christian, et al. "Limited evidence of chondrocyte outgrowth from adult human articular cartilage." Osteoarthritis and Cartilage 24.1 (2016): 124-8.
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