Proteomic Analysis of a Fraction with Intact Eyespots of Chlamydomonas reinhardtii and Assignment of Protein Methylation

Journal article


Publication Details

Author(s): Eitzinger N, Wagner V, Weisheit W, Geimer S, Boness D, Kreimer G, Mittag M
Journal: Frontiers in Plant Science
Publisher: Frontiers Media
Publication year: 2015
Volume: 6
ISSN: 1664-462X


Abstract


Flagellate green algae possess a visual system, the eyespot. In Chlamydomonas reinhardtii it is situated at the edge of the chloroplast and consists of two carotenoid rich lipid globule layers subtended by thylakoid membranes (TM) that are attached to both chloroplast envelope membranes and a specialized area of the plasma membrane (PM). A former analysis of an eyespot fraction identified 203 proteins. To increase the understanding of eyespot related processes, knowledge of the protein composition of the membranes in its close vicinity is desirable. Here, we present a purification procedure that allows isolation of intact eyespots. This gain in intactness goes, however, hand in hand with an increase of contaminants from other organelles. Proteomic analysis identified 742 proteins. Novel candidates include proteins for eyespot development, retina-related proteins, ion pumps, and membrane-associated proteins, calcium sensing proteins as well as kinases, phosphatases and 14-3-3 proteins. Methylation of proteins at Arg or Lys is known as an important posttranslational modification involved in, e.g., signal transduction. Here, we identify several proteins from eyespot fractions that are methylated at Arg and/or Lys. Among them is the eyespot specific SOUL3 protein that influences the size and position of the eyespot and EYE2, a protein important for its development.



FAU Authors / FAU Editors

Boness, David
Lehrstuhl für Zellbiologie
Eitzinger, Nicole
Medizinische Fakultät
Kreimer, Georg Prof. Dr.
Professur für Zellbiologie


How to cite

APA:
Eitzinger, N., Wagner, V., Weisheit, W., Geimer, S., Boness, D., Kreimer, G., & Mittag, M. (2015). Proteomic Analysis of a Fraction with Intact Eyespots of Chlamydomonas reinhardtii and Assignment of Protein Methylation. Frontiers in Plant Science, 6. https://dx.doi.org/10.3389/fpls.2015.01085

MLA:
Eitzinger, Nicole, et al. "Proteomic Analysis of a Fraction with Intact Eyespots of Chlamydomonas reinhardtii and Assignment of Protein Methylation." Frontiers in Plant Science 6 (2015).

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Last updated on 2018-19-04 at 03:40