Serum-induced keratinization processes in an immortalized human meibomian gland epithelial cell line

Hampel U, Schröder A, Mitchell T, Brown S, Snikeris P, Garreis F, Kunnen C, Willcox M, Paulsen F (2015)

Publication Type: Journal article

Publication year: 2015

Journal

PLoS ONE Public Library of Science

Book Volume: 10

Pages Range: e0128096

Journal Issue: 6

DOI: 10.1371/journal.pone.0128096

Abstract

The aim of this study was to evaluate a human meibomian gland epithelial cell line (HMGEC) as a model for meibomian gland (patho)physiology in vitro.HMGEC were cultured in the absence or presence of serum. Sudan III lipid staining, ultrastructural analysis and lipidomic analyses were performed. Impedance sensing, desmoplakin 1/2 mRNA and cytokeratin (CK) 1, 5, 6, 14 levels were evaluated. Serum containing medium supplemented with higher serum, glucose, an omega-3 lipid cocktail, eicosapentaenoic acid or sebomed medium were investigated for lipid accumulation and ultrastructural morphology.Lipid droplet accumulation in HMGEC was induced by serum containing media after 1 day, but decreased over time. Cultivation in serum induced desmosome and cytokeratin filament formation. Desmoplakin 1/2 gene levels were significantly upregulated after 1d of serum treatment. Furthermore, the normalized impedance increased significantly. Lipidome analysis revealed high levels of phospholipids (over 50%), but very low levels of wax ester and cholesteryl esters (under 1%). Stimulation with eicosapentaenoic acid increased lipid accumulation after one day.Serum treatment of HMGEC caused lipid droplet formation to some extent but also induced keratinization. The cells did not produce typical meibum lipids under these growth conditions. HMGEC are well suited to study (hyper)keratinization processes of meibomian gland epithelial cells in vitro.

Authors with CRIS profile

Ulrike Hampel Lehrstuhl für Funktionelle und Klinische Anatomie Antje Schröder Lehrstuhl für Funktionelle und Klinische Anatomie Fabian Garreis Lehrstuhl für Funktionelle und Klinische Anatomie Friedrich Paulsen Lehrstuhl für Funktionelle und Klinische Anatomie

Involved external institutions

University of Wollongong (UOW) AU Australia (AU) University of New South Wales (UNSW) AU Australia (AU)

How to cite

APA:

Hampel, U., Schröder, A., Mitchell, T., Brown, S., Snikeris, P., Garreis, F.,... Paulsen, F. (2015). Serum-induced keratinization processes in an immortalized human meibomian gland epithelial cell line. PLoS ONE, 10(6), e0128096. https://dx.doi.org/10.1371/journal.pone.0128096

MLA:

Hampel, Ulrike, et al. "Serum-induced keratinization processes in an immortalized human meibomian gland epithelial cell line." PLoS ONE 10.6 (2015): e0128096.

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