Is non-buffered DMEM solution a suitable medium for in vitro bioactivity tests?

Rohanova D, Boccaccini AR, Horkavcova D, Bozdechova P, Bezdicka P, Castoralova M (2014)

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2014

Journal

Journal of Materials Chemistry B Royal Society of Chemistry

Book Volume: 2

Pages Range: 5068-5076

Journal Issue: 31

DOI: 10.1039/c4tb00187g

Abstract

Several laboratories had tested bioactivity of the materials in commercially available solution DMEM (Dulbecco's Modified Eagle's Medium) that is normally used for cultivation of cell cultures. The objective of this work was to find out whether it is possible to replace TRIS-buffered SBF currently used for bioactivity tests with the non-buffered DMEM solution. To understand the role of the organic part of the DMEM solution in the process of crystallization, we have prepared non-buffered solution simulating only its inorganic part (identified as I-solution). It was found that under static-dynamic test conditions calcite (CaCO) and the amorphous phase of calcium phosphate (ACP) formed on the surface of the glass-ceramic (45S5 bioactive glass based) scaffold exposed to both solutions. Additionally, halite (NaCl) formed at the beginning of exposure to DMEM. Hydroxyapatite phase was not detected on the surface in either non-buffered solution. Organic components contained in the DMEM solution failed to prevent formation of crystalline phases. The present results indicate that it is not recommendable to use DMEM for bioactivity tests of glass-ceramic materials due to its low concentration of Ca ions, high concentration of HCO ions and the necessity to maintain sterile environment during the test. © 2014 the Partner Organisations.

Authors with CRIS profile

Aldo Boccaccini Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)

Involved external institutions

Academy of Sciences of the Czech Republic (ASCR) / Akademie věd České republiky (AVČR) CZ Czech Republic (CZ) University of Chemistry and Technology (UCT) / Vysoká škola chemicko-technologická (VŠCHT) CZ Czech Republic (CZ)

How to cite

APA:

Rohanova, D., Boccaccini, A.R., Horkavcova, D., Bozdechova, P., Bezdicka, P., & Castoralova, M. (2014). Is non-buffered DMEM solution a suitable medium for in vitro bioactivity tests? Journal of Materials Chemistry B, 2(31), 5068-5076. https://dx.doi.org/10.1039/c4tb00187g

MLA:

Rohanova, Dana, et al. "Is non-buffered DMEM solution a suitable medium for in vitro bioactivity tests?" Journal of Materials Chemistry B 2.31 (2014): 5068-5076.

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