Different storage conditions influence biocompatibility and physicochemical properties of iron oxide nanoparticles

Journal article
(Original article)


Publication Details

Author(s): Zaloga J, Janko C, Agarwal R, Nowak J, Müller R, Boccaccini AR, Lee G, Odenbach S, Lyer S, Alexiou C
Journal: International Journal of Molecular Sciences
Publisher: MDPI
Publication year: 2015
Volume: 16
Journal issue: 5
Pages range: 9368-9384
ISSN: 1422-0067


Abstract


Superparamagnetic iron oxide nanoparticles (SPIONs) have attracted increasing attention in many biomedical fields. In magnetic drug targeting SPIONs are injected into a tumour supplying artery and accumulated inside the tumour with a magnet. The effectiveness of this therapy is thus dependent on magnetic properties, stability and biocompatibility of the particles. A good knowledge of the effect of storage conditions on those parameters is of utmost importance for the translation of the therapy concept into the clinic and for reproducibility in preclinical studies. Here, core shell SPIONs with a hybrid coating consisting of lauric acid and albumin were stored at different temperatures from 4 to 45 °C over twelve weeks and periodically tested for their physicochemical properties over time. Surprisingly, even at the highest storage temperature we did not observe denaturation of the protein or colloidal instability. However, the saturation magnetisation decreased by maximally 28.8% with clear correlation to time and storage temperature. Furthermore, the biocompatibility was clearly affected, as cellular uptake of the SPIONs into human T-lymphoma cells was crucially dependent on the storage conditions. Taken together, the results show that the particle properties undergo significant changes over time depending on the way they are stored.



FAU Authors / FAU Editors

Alexiou, Christoph Prof. Dr.
Professur für Nanomedizin
Boccaccini, Aldo R. Prof. Dr.-Ing.
Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)
Janko, Christina Dr. rer. nat.
Lehrstuhl für Genetik
Lee, Geoffrey Prof.
Lehrstuhl für Pharmazeutische Technologie
Lyer, Stefan Dr. rer. nat.
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie
Zaloga, Jan Dr. rer. nat.
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie


Additional Organisation
Exzellenz-Cluster Engineering of Advanced Materials


External institutions with authors

Leibniz-Institut für Photonische Technologien e.V.
Technische Universität Dresden


Research Fields

Nanosafety
Exzellenz-Cluster Engineering of Advanced Materials


How to cite

APA:
Zaloga, J., Janko, C., Agarwal, R., Nowak, J., Müller, R., Boccaccini, A.R.,... Alexiou, C. (2015). Different storage conditions influence biocompatibility and physicochemical properties of iron oxide nanoparticles. International Journal of Molecular Sciences, 16(5), 9368-9384. https://dx.doi.org/10.3390/ijms16059368

MLA:
Zaloga, Jan, et al. "Different storage conditions influence biocompatibility and physicochemical properties of iron oxide nanoparticles." International Journal of Molecular Sciences 16.5 (2015): 9368-9384.

BibTeX: 

Last updated on 2019-18-07 at 07:33