ImmunoPET/MR imaging allows specific detection of Aspergillus fumigatus lung infection in vivo

Journal article


Publication Details

Author(s): Rolle AM, Hasenberg M, Thornton CR, Solouk-Saran D, Maenn L, Weski J, Maurer A, Fischer E, Spycher PR, Schibli R, Boschetti F, Stegemann-Koniszewski S, Bruder D, Severin GW, Autenrieth SE, Krappmann S, Davies G, Pichler BJ, Gunzer M, Wiehr S
Journal: Proceedings of the National Academy of Sciences of the United States of America
Publication year: 2016
Volume: 113
Journal issue: 8
Pages range: E1026-33
ISSN: 0027-8424


Abstract

Invasive pulmonary aspergillosis (IPA) is a life-threatening lung disease caused by the fungus Aspergillus fumigatus, and is a leading cause of invasive fungal infection-related mortality and morbidity in patients with hematological malignancies and bone marrow transplants. We developed and tested a novel probe for noninvasive detection of A. fumigatus lung infection based on antibody-guided positron emission tomography and magnetic resonance (immunoPET/MR) imaging. Administration of a [(64)Cu]DOTA-labeled A. fumigatus-specific monoclonal antibody (mAb), JF5, to neutrophil-depleted A. fumigatus-infected mice allowed specific localization of lung infection when combined with PET. Optical imaging with a fluorochrome-labeled version of the mAb showed colocalization with invasive hyphae. The mAb-based newly developed PET tracer [(64)Cu]DOTA-JF5 distinguished IPA from bacterial lung infections and, in contrast to [(18)F]FDG-PET, discriminated IPA from a general increase in metabolic activity associated with lung inflammation. To our knowledge, this is the first time that antibody-guided in vivo imaging has been used for noninvasive diagnosis of a fungal lung disease (IPA) of humans, an approach with enormous potential for diagnosis of infectious diseases and with potential for clinical translation.


FAU Authors / FAU Editors

Krappmann, Sven Prof. Dr.
Professur für Klinische Mikrobiologie und Immunologie


External institutions with authors

Eberhard Karls Universität Tübingen
Helmholtz-Zentrum für Infektionsforschung (HZI)
Paul Scherrer Institute (PSI)
Technical University of Denmark / Danmarks Tekniske Universitet (DTU)
Universität Duisburg-Essen (UDE)
Universitätsklinikum Tübingen
Université Bourgogne Franche-Comté
University of Exeter


How to cite

APA:
Rolle, A.-M., Hasenberg, M., Thornton, C.R., Solouk-Saran, D., Maenn, L., Weski, J.,... Wiehr, S. (2016). ImmunoPET/MR imaging allows specific detection of Aspergillus fumigatus lung infection in vivo. Proceedings of the National Academy of Sciences of the United States of America, 113(8), E1026-33. https://dx.doi.org/10.1073/pnas.1518836113

MLA:
Rolle, Anna-Maria, et al. "ImmunoPET/MR imaging allows specific detection of Aspergillus fumigatus lung infection in vivo." Proceedings of the National Academy of Sciences of the United States of America 113.8 (2016): E1026-33.

BibTeX: 

Last updated on 2018-10-10 at 08:22