ImmunoPET/MR imaging allows specific detection of Aspergillus fumigatus lung infection in vivo

Rolle AM, Hasenberg M, Thornton CR, Solouk-Saran D, Maenn L, Weski J, Maurer A, Fischer E, Spycher PR, Schibli R, Boschetti F, Stegemann-Koniszewski S, Bruder D, Severin GW, Autenrieth SE, Krappmann S, Davies G, Pichler BJ, Gunzer M, Wiehr S (2016)

Publication Type: Journal article

Publication year: 2016

Journal

Proceedings of the National Academy of Sciences of the United States of America National Academy of Sciences

Book Volume: 113

Pages Range: E1026-33

Journal Issue: 8

DOI: 10.1073/pnas.1518836113

Abstract

Invasive pulmonary aspergillosis (IPA) is a life-threatening lung disease caused by the fungus Aspergillus fumigatus, and is a leading cause of invasive fungal infection-related mortality and morbidity in patients with hematological malignancies and bone marrow transplants. We developed and tested a novel probe for noninvasive detection of A. fumigatus lung infection based on antibody-guided positron emission tomography and magnetic resonance (immunoPET/MR) imaging. Administration of a [(64)Cu]DOTA-labeled A. fumigatus-specific monoclonal antibody (mAb), JF5, to neutrophil-depleted A. fumigatus-infected mice allowed specific localization of lung infection when combined with PET. Optical imaging with a fluorochrome-labeled version of the mAb showed colocalization with invasive hyphae. The mAb-based newly developed PET tracer [(64)Cu]DOTA-JF5 distinguished IPA from bacterial lung infections and, in contrast to [(18)F]FDG-PET, discriminated IPA from a general increase in metabolic activity associated with lung inflammation. To our knowledge, this is the first time that antibody-guided in vivo imaging has been used for noninvasive diagnosis of a fungal lung disease (IPA) of humans, an approach with enormous potential for diagnosis of infectious diseases and with potential for clinical translation.

Authors with CRIS profile

Sven Krappmann Professur für Klinische Mikrobiologie und Immunologie

Involved external institutions

Eberhard Karls Universität Tübingen DE Germany (DE) Universität Duisburg-Essen (UDE) DE Germany (DE) University of Exeter GB United Kingdom (GB) Universitätsklinikum Tübingen DE Germany (DE) Technical University of Denmark / Danmarks Tekniske Universitet (DTU) DK Denmark (DK) Helmholtz-Zentrum für Infektionsforschung (HZI) DE Germany (DE) Université Bourgogne Franche-Comté FR France (FR) Paul Scherrer Institute (PSI) CH Switzerland (CH)

How to cite

APA:

Rolle, A.-M., Hasenberg, M., Thornton, C.R., Solouk-Saran, D., Maenn, L., Weski, J.,... Wiehr, S. (2016). ImmunoPET/MR imaging allows specific detection of Aspergillus fumigatus lung infection in vivo. Proceedings of the National Academy of Sciences of the United States of America, 113(8), E1026-33. https://dx.doi.org/10.1073/pnas.1518836113

MLA:

Rolle, Anna-Maria, et al. "ImmunoPET/MR imaging allows specific detection of Aspergillus fumigatus lung infection in vivo." Proceedings of the National Academy of Sciences of the United States of America 113.8 (2016): E1026-33.

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