The Different Binding Properties of Cultured Human Corneal Endothelial Cell Subpopulations to Descemet's Membrane Components

Beitrag in einer Fachzeitschrift


Details zur Publikation

Autor(en): Toda M, Ueno M, Yamada J, Hiraga A, Tanaka H, Schlötzer-Schrehardt U, Sotozono C, Kinoshita S, Hamuro J
Zeitschrift: Investigative Ophthalmology & Visual Science
Jahr der Veröffentlichung: 2016
Band: 57
Heftnummer: 11
Seitenbereich: 4599-605
ISSN: 0146-0404


Abstract


To clarify the adherent properties of cultured human corneal endothelial cell (cHCEC) subpopulations (SPs).Each SP was prepared by controlling the culture conditions or by using magnetic cell separation, and then confirmed by staining with several cell-surface markers. Binding abilities of HCEC SPs were examined by adding the cells to culture plates immobilized with collagens, laminins, or proteoglycans, and then centrifuging the plates. Adhered cells were then evaluated by phase-contrast microscopy.The cHCECs were bound to laminin-511, laminin-411, and Type-IV collagen in a concentration-dependent manner, yet weakly bound to Perlecan, Agrin, and TSP-1. Comparison of the influence of cell-suspension vehicles on cHCEC attachment showed that cells suspended in Opti-MEM-I or Opeguard-MA were bound to laminin, yet no binding was observed in cells suspended in BSS-Plus. Next, we compared the adherent properties of HCEC SPs. Both the fully differentiated, mature cHCEC SP and the epithelial-to-mesenchymal-transitioned (EMT)-phenotype SP were found to attach to laminin- or collagen-coated plates. Interestingly, the binding properties to laminins differed among those SPs. Although the level of cells adhered to the laminin-411-coated plate was the same among the cHCEC SPs, the fully differentiated, mature cHCEC SP was significantly more tightly bound to laminin-511 than was the EMT-phenotype SP.The findings of this study suggest that the binding ability of cHCECs to major Descemet's membrane components is distinct among cHCEC SPs, and that Opti-MEM-I and Opeguard-MA are useful cell-suspension vehicles for cell-injection therapy.



FAU-Autoren / FAU-Herausgeber

Schlötzer-Schrehardt, Ursula apl. Prof. Dr.
Medizinische Fakultät


Zusätzliche Organisationseinheit(en)
Augenklinik


Autor(en) der externen Einrichtung(en)
Kyoto Prefectural University of Medicine / 京都府立医科大学


Zitierweisen

APA:
Toda, M., Ueno, M., Yamada, J., Hiraga, A., Tanaka, H., Schlötzer-Schrehardt, U.,... Hamuro, J. (2016). The Different Binding Properties of Cultured Human Corneal Endothelial Cell Subpopulations to Descemet's Membrane Components. Investigative Ophthalmology & Visual Science, 57(11), 4599-605. https://dx.doi.org/10.1167/iovs.16-20087

MLA:
Toda, Munetoyo, et al. "The Different Binding Properties of Cultured Human Corneal Endothelial Cell Subpopulations to Descemet's Membrane Components." Investigative Ophthalmology & Visual Science 57.11 (2016): 4599-605.

BibTeX: 

Zuletzt aktualisiert 2018-10-10 um 08:19