Identification of Novel STAT6-Regulated Proteins in Mouse B Cells by Comparative Transcriptome and Proteome Analysis

Mokada Gopal L, Boeser A, Lehmann C, Drepper F, Dudziak D, Warscheid B, Vöhringer D (2017)

Publication Type: Journal article

Publication year: 2017

Journal

Book Volume: 198

Pages Range: 3737-3745

Journal Issue: 9

DOI: 10.4049/jimmunol.1601838

Abstract

The transcription factor STAT6 plays a key role in mediating signaling downstream of the receptors for IL-4 and IL-13. In B cells, STAT6 is required for class switch recombination to IgE and for germinal center formation during type 2 immune responses directed against allergens or helminths. In this study, we compared the transcriptomes and proteomes of primary mouse B cells from wild-type and STAT6-deficient mice cultured for 4 d in the presence or absence of IL-4. Microarray analysis revealed that 214 mRNAs were upregulated and 149 were downregulated >3-fold by IL-4 in a STAT6-dependent manner. Across all samples, ~5000 proteins were identified by label-free quantitative liquid chromatography/mass spectrometry. A total of 149 proteins was found to be differentially expressed >3-fold between IL-4-stimulated wild-type and STAT6(-/-) B cells (75 upregulated and 74 downregulated). Comparative analysis of the proteome and transcriptome revealed that expression of these proteins was mainly regulated at the transcriptional level, which argues against a major role for posttranscriptional mechanisms that modulate the STAT6-dependent proteome. Nine proteins were selected for confirmation by flow cytometry or Western blot. We show that CD30, CD79b, SLP-76, DEC205, IL-5R?, STAT5, and Thy1 are induced by IL-4 in a STAT6-dependent manner. In contrast, Syk and Fc receptor-like 1 were downregulated. This dataset provides a framework for further functional analysis of newly identified IL-4-regulated proteins in B cells that may contribute to germinal center formation and IgE switching in type 2 immunity.

Authors with CRIS profile

Additional Organisation(s)

Involved external institutions

Albert-Ludwigs-Universität Freiburg Germany (DE)

How to cite

APA:

Mokada Gopal, L., Boeser, A., Lehmann, C., Drepper, F., Dudziak, D., Warscheid, B., & Vöhringer, D. (2017). Identification of Novel STAT6-Regulated Proteins in Mouse B Cells by Comparative Transcriptome and Proteome Analysis. Journal of Immunology, 198(9), 3737-3745. https://dx.doi.org/10.4049/jimmunol.1601838

MLA:

Mokada Gopal, Lavanya, et al. "Identification of Novel STAT6-Regulated Proteins in Mouse B Cells by Comparative Transcriptome and Proteome Analysis." Journal of Immunology 198.9 (2017): 3737-3745.

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