Beta-arrestin is a necessary component of Wnt/beta-catenin signaling in vitro and in vivo.

Bryja V, Gradl D, Schambony A, Arenas E, Schulte G (2007)

Publication Type: Journal article, Original article

Subtype: other

Publication year: 2007

Journal

Proceedings of the National Academy of Sciences of the United States of America National Academy of Sciences

Book Volume: 104

Pages Range: 6690-6695

Journal Issue: 16

URI: http://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=17426148&retmode=ref&cmd=prlinks

DOI: 10.1073/pnas.0611356104

Abstract

The Wnt/beta-catenin signaling pathway is crucial for proper embryonic development and tissue homeostasis. The phosphoprotein dishevelled (Dvl) is an integral part of Wnt signaling and has recently been shown to interact with the multifunctional scaffolding protein beta-arrestin. Using Dvl deletion constructs, we found that beta-arrestin binds a region N-terminal of the PDZ domain of Dvl, which contains casein kinase 1 (CK1) phosphorylation sites. Inhibition of Wnt signaling by CK1 inhibitors reduced the binding of beta-arrestin to Dvl. Moreover, mouse embryonic fibroblasts lacking beta-arrestins were able to phosphorylate LRP6 in response to Wnt-3a but decreased the activation of Dvl and blocked beta-catenin signaling. In addition, we found that beta-arrestin can bind axin and forms a trimeric complex with axin and Dvl. Furthermore, treatment of Xenopus laevis embryos with beta-arrestin morpholinos reduced the activation of endogenous beta-catenin, decreased the expression of the beta-catenin target gene, Xnr3, and blocked axis duplication induced by X-Wnt-8, CK1epsilon, or DshDeltaDEP, but not by beta-catenin. Thus, our results identify beta-arrestin as a necessary component for Wnt/beta-catenin signaling, linking Dvl and axin, and open a vast array of signaling avenues and possibilities for cross-talk with other beta-arrestin-dependent signaling pathways.

Authors with CRIS profile

Alexandra Schambony Lehrstuhl für Entwicklungsbiologie

Involved external institutions

Karlsruhe Institute of Technology (KIT) DE Germany (DE) Karolinska Institute SE Sweden (SE)

How to cite

APA:

Bryja, V., Gradl, D., Schambony, A., Arenas, E., & Schulte, G. (2007). Beta-arrestin is a necessary component of Wnt/beta-catenin signaling in vitro and in vivo. Proceedings of the National Academy of Sciences of the United States of America, 104(16), 6690-6695. https://dx.doi.org/10.1073/pnas.0611356104

MLA:

Bryja, Vitezslav, et al. "Beta-arrestin is a necessary component of Wnt/beta-catenin signaling in vitro and in vivo." Proceedings of the National Academy of Sciences of the United States of America 104.16 (2007): 6690-6695.

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