A hypomorphic IgH-chain allele affects development of B-cell subsets and favours receptor editing
Brenner S, Drewel D, Steinbart T, Weisel F, Härtel E, Pötzsch S, Welzel H, Brandl A, Yu P, Mudde GC, Schweizer A, Nitschke L, Winkler T (2011)
Publication Status: Published
Publication Type: Journal article
Publication year: 2011
Journal
Publisher: NATURE PUBLISHING GROUP
Book Volume: 30
Pages Range: 2705-2718
Journal Issue: 13
Abstract
The quality and quantity of BCR signals impact on cell fate decisions of B lymphocytes. Here, we describe novel gene-targeted mice, which in the context of normal VDJ recombination show hypomorphic expression of immunoglobulin l heavy chain (mu HC) mRNA levels and hence lower pre-BCR and BCR levels. Hypomorphic expression of mu HC leads to augmented selection processes at all stages of B-cell development, noticeably at the expansion of pre-B cells, the positive selection of immature B lymphocytes in the bone marrow and the selection of the follicular (FO), marginal zone (MZ) and B1 B-lymphocyte compartment in peripheral lymphoid organs. Immature as well as mature FO and MZ B lymphocytes in the peripheral lymphoid organs express lower levels of the receptor for B-cell activating factor (BAFF). In addition, hypomorphic expression of the BCR favours receptor editing. Together, our results highlight the critical importance of pre-BCR and BCR receptor levels for the normal development of B-lymphocyte subpopulations in the context of intact VDJ recombination and a diverse antibody repertoire. The EMBO Journal (2011) 30, 2705-2718. doi:10.1038/emboj.2011.168; Published online 27 May 2011
Authors with CRIS profile
Sonja Pötzsch
Institute of Microbiology – Clinical Microbiology, Immunology and Hygiene
Astrid Schweizer
Lars Nitschke Professur für Genetik Thomas Winkler Professur für Genetik
Lars Nitschke Professur für Genetik Thomas Winkler Professur für Genetik
Involved external institutions
Germany (DE)How to cite
APA:
Brenner, S., Drewel, D., Steinbart, T., Weisel, F., Härtel, E., Pötzsch, S.,... Winkler, T. (2011). A hypomorphic IgH-chain allele affects development of B-cell subsets and favours receptor editing. EMBO Journal, 30(13), 2705-2718. https://dx.doi.org/10.1038/emboj.2011.168
MLA:
Brenner, Sven, et al. "A hypomorphic IgH-chain allele affects development of B-cell subsets and favours receptor editing." EMBO Journal 30.13 (2011): 2705-2718.
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