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Two novel disaccharides, rutinose and methylrutinose, are involved in carbon metabolism in Datisca glomerata

Schubert M, Melnikova AN, Mesecke N, Zubkova EK, Fortte R, Batashev DR, Barth I, Sauer N, Gamalei YV, Mamushina NS, Tietze LF, Voitsekhovskaja OV, Pawlowski K (2010)

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Publication Language: English

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2010

Journal

Book Volume: 231

Pages Range: 507-521

Journal Issue: 3

DOI: 10.1007/s00425-009-1049-5

Open Access Link: http://link.springer.com/article/10.1007%2Fs00425-009-1049-5

Abstract

Datisca glomerata forms nitrogen-fixing root nodules in symbiosis with soil actinomycetes from the genus Frankia. Analysis of sugars in roots, nodules and leaves of D. glomerata revealed the presence of two novel compounds that were identified as α-l-rhamnopyranoside-(1 → 6)-d-glucose (rutinose) and α-l-rhamnopyranoside-(1 → 6)-1-O-β-d-methylglucose (methylrutinose). Rutinose has been found previously as a/the glycoside part of several flavonoid glycosides, e. g. rutin, also of datiscin, the main flavonoid of Datisca cannabina, but had not been reported as free sugar. Time course analyses suggest that both rutinose and methylrutinose might play a role in transient carbon storage in sink organs and, to a lesser extent, in source leaves. Their concentrations show that they can accumulate in the vacuole. Rutinose, but not methylrutinose, was accepted as a substrate by the tonoplast disaccharide transporter SUT4 from Arabidopsis. In vivo C-labeling and the study of uptake of exogenous sucrose and rutinose from the leaf apoplast showed that neither rutinose nor methylrutinose appreciably participate in phloem translocation of carbon from source to sink organs, despite rutinose being found in the apoplast at significant levels. A model for sugar metabolism in D. glomerata is presented. © 2009 The Author(s).

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How to cite

APA:

Schubert, M., Melnikova, A.N., Mesecke, N., Zubkova, E.K., Fortte, R., Batashev, D.R.,... Pawlowski, K. (2010). Two novel disaccharides, rutinose and methylrutinose, are involved in carbon metabolism in Datisca glomerata. Planta, 231(3), 507-521. https://dx.doi.org/10.1007/s00425-009-1049-5

MLA:

Schubert, Maria, et al. "Two novel disaccharides, rutinose and methylrutinose, are involved in carbon metabolism in Datisca glomerata." Planta 231.3 (2010): 507-521.

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