Nanoparticles for intravascular applications: physicochemical characterization and cytotoxicity testing

Matuszak J, Baumgartner J, Zaloga J, Juenet M, Da Silva AE, Franke D, Almer G, Texier I, Faivre D, Metselaar JM, Navarro FP, Chauvierre C, Prassl R, Dezsi L, Urbanics R, Alexiou C, Mangge H, Szebeni J, Letourneur D, Cicha I (2016)

Publication Type: Journal article

Publication year: 2016

Journal

Nanomedicine Future Medicine

Book Volume: 11

Pages Range: 597-616

Journal Issue: 6

DOI: 10.2217/nnm.15.216

Abstract

We report the physicochemical analysis of nanosystems intended for cardiovascular applications and their toxicological characterization in static and dynamic cell culture conditions.Size, polydispersity and ?-potential were determined in 10 nanoparticle systems including liposomes, lipid nanoparticles, polymeric and iron oxide nanoparticles. Nanoparticle effects on primary human endothelial cell viability were monitored using real-time cell analysis and live-cell microscopy in static conditions, and in a flow model of arterial bifurcations.The majority of tested nanosystems were well tolerated by endothelial cells up to the concentration of 100 ?g/ml in static, and up to 400 ?g/ml in dynamic conditions. Pilot experiments in a pig model showed that intravenous administration of liposomal nanoparticles did not evoke the hypersensitivity reaction. These findings are of importance for future clinical use of nanosystems intended for intravascular applications.

Authors with CRIS profile

Jasmin Matuszak Department of Otorhinolaryngology – Head and Neck Surgery Jan Zaloga Department of Otorhinolaryngology – Head and Neck Surgery Christoph Alexiou Professur für Nanomedizin Iwona Cicha Lehrstuhl für Innere Medizin II

Involved external institutions

University of Paris 13 - Paris-Nord / Université Paris XIII Paris-Nord FR France (FR) Semmelweis University / Semmelweis Egyetem HU Hungary (HU) SeroScience Ltd HU Hungary (HU) University of Grenoble Alpes (UGA) / Université de Grenoble FR France (FR) University of Twente NL Netherlands (NL) Max-Planck-Institut für Kolloid- und Grenzflächenforschung / Max Planck Institute of Colloids and Interfaces DE Germany (DE) nanoPET Pharma GmbH DE Germany (DE)

How to cite

APA:

Matuszak, J., Baumgartner, J., Zaloga, J., Juenet, M., Da Silva, A.E., Franke, D.,... Cicha, I. (2016). Nanoparticles for intravascular applications: physicochemical characterization and cytotoxicity testing. Nanomedicine, 11(6), 597-616. https://dx.doi.org/10.2217/nnm.15.216

MLA:

Matuszak, Jasmin, et al. "Nanoparticles for intravascular applications: physicochemical characterization and cytotoxicity testing." Nanomedicine 11.6 (2016): 597-616.

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