Induction of proliferation and pro-inflammatory cytokine production in rheumatoid arthritis peripheral blood mononuclear cells by a 65 KDa chondrocyte membrane-specific, constitutive target autoantigen (CH65)

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Details zur Publikation

Autor(en): Alsalameh RJ, Casey RC, Mollenhauer J, Kalden J, Burmester GR, Alsalameh SM
Zeitschrift: International Journal of Rheumatic Diseases
Jahr der Veröffentlichung: 2013
ISSN: 1756-1841


Abstract


To analyze proliferation and pro-inflammatory cytokine production of peripheral blood mononuclear cells (PBMC) from rheumatoid arthritis (RA) patients following stimulation with a purified chondrocyte membrane-associated autoantigen (CH65).CH65 was highly purified from bovine chondrocyte membranes by solubilization and ion exchange chromatography. PBMC of RA patients (n = 37; 28 seropositive, nine seronegative) and non-arthritic donors (n = 20) were isolated by ficoll centrifugation and used in cell proliferation assays. The levels of interleukin (IL)-1, tumo necrosis factor (TNF) and IL-6 produced after stimulation with CH65 were determined by enzyme-linked immunosorbent assay (ELISA). Statistical analysis was performed using Mann-Whitney U-test and Spearman rank test and the software SPSS 13.0(TM) (SPSS Inc.; Chicago, IL, USA).Peripheral blood mononuclear cells exhibited a strong proliferative response to purified CH65 in approximately 50% of the RA patients (seropositive > seronegative), with a maximum reactivity at 0.15 or 0.30 ?g/mL culture medium. In contrast, PBMC from normal donors did not show a proliferative response to CH65 at any dose. The proliferative response in RA patients peaked at days 7-9 and returned to control levels at day 13, indicating an antigen-driven process. CH65-stimulated RA PBMC produced moderate to high amounts of IL-1, TNF and IL-6. This was comparable to the response after exposure to isolated whole chondrocyte membranes or purified collagen type II.These results demonstrate a significant cellular immune response to CH65 protein in RA patients. Given the high similarity between bovine and human CH65, the results suggest a pathogenetic involvement of this molecule as a cartilage-specific potential target autoantigen in RA.



FAU-Autoren / FAU-Herausgeber

Kalden, Joachim Prof. Dr.
Medizinische Fakultät


Autor(en) der externen Einrichtung(en)
Charité - Universitätsmedizin Berlin
Rush University


Zitierweisen

APA:
Alsalameh, R.J., Casey, R.C., Mollenhauer, J., Kalden, J., Burmester, G.R., & Alsalameh, S.M. (2013). Induction of proliferation and pro-inflammatory cytokine production in rheumatoid arthritis peripheral blood mononuclear cells by a 65 KDa chondrocyte membrane-specific, constitutive target autoantigen (CH65). International Journal of Rheumatic Diseases. https://dx.doi.org/10.1111/1756-185X.12167

MLA:
Alsalameh, Rayya J., et al. "Induction of proliferation and pro-inflammatory cytokine production in rheumatoid arthritis peripheral blood mononuclear cells by a 65 KDa chondrocyte membrane-specific, constitutive target autoantigen (CH65)." International Journal of Rheumatic Diseases (2013).

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Zuletzt aktualisiert 2018-10-10 um 02:25