Catchup: a mouse model for imaging-based tracking and modulation of neutrophil granulocytes.

Hasenberg A, Hasenberg M, Maenn L, Neumann F, Borkenstein L, Stecher M, Kraus A, Engel DR, Klingberg A, Seddigh P, Abdullah Z, Klebow S, Engelmann S, Reinhold A, Brandau S, Seeling M, Waisman A, Schraven B, Goethert JR, Nimmerjahn F, Gunzer M (2015)

Publication Status: Published

Publication Type: Journal article

Publication year: 2015

Journal

Nature methods

Book Volume: 12

Pages Range: 445-52

Journal Issue: 5

DOI: 10.1038/nmeth.3322

Abstract

Neutrophil granulocyte biology is a central issue of immunological research, but the lack of animal models that allow for neutrophil-selective genetic manipulation has delayed progress. By modulating the neutrophil-specific locus Ly6G with a knock-in allele expressing Cre recombinase and the fluorescent protein tdTomato, we generated a mouse model termed Catchup that exhibits strong neutrophil specificity. Transgene activity was found only in very few eosinophils and basophils and was undetectable in bone marrow precursors, including granulomonocytic progenitors (GMPs). Cre-mediated reporter-gene activation allowed for intravital two-photon microscopy of neutrophils without adoptive transfer. Homozygous animals were Ly6G deficient but showed normal leukocyte cellularity in all measured organs. Ly6G-deficient neutrophils were functionally normal in vitro and in multiple models of sterile or infectious inflammation in vivo. However, Cre-mediated deletion of FcγRIV in neutrophils reduced the cells' recruitment to immune-complex-mediated peritonitis, suggesting a cell-intrinsic role for activating Fc receptors in neutrophil trafficking.

Authors with CRIS profile

Michaela Seeling Lehrstuhl für Genetik Falk Nimmerjahn Lehrstuhl für Genetik

Involved external institutions

Universität Duisburg-Essen (UDE) DE Germany (DE) Rheinische Friedrich-Wilhelms-Universität Bonn DE Germany (DE) Johannes Gutenberg-Universität Mainz (JGU) DE Germany (DE) Otto-von-Guericke-Universität Magdeburg DE Germany (DE)

How to cite

APA:

Hasenberg, A., Hasenberg, M., Maenn, L., Neumann, F., Borkenstein, L., Stecher, M.,... Gunzer, M. (2015). Catchup: a mouse model for imaging-based tracking and modulation of neutrophil granulocytes. Nature methods, 12(5), 445-52. https://doi.org/10.1038/nmeth.3322

MLA:

Hasenberg, Anja, et al. "Catchup: a mouse model for imaging-based tracking and modulation of neutrophil granulocytes." Nature methods 12.5 (2015): 445-52.

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