Extensive regulation of the non-coding transcriptome by hypoxia: role of HIF in releasing paused RNApol2
Choudhry H, Schoedel J, Oikonomopoulos S, Camps C, Grampp S, Harris AL, Ratcliffe PJ, Ragoussis J, Mole DR (2014)
Publication Type: Journal article
Publication year: 2014
Journal
Book Volume: 15
Pages Range: 70-6
Journal Issue: 1
Abstract
Hypoxia is central to both ischaemic and neoplastic diseases. However, the non-coding transcriptional response to hypoxia is largely uncharacterized. We undertook integrated genomic analyses of both non-coding and coding transcripts using massively parallel sequencing and interfaced this data with pan-genomic analyses of hypoxia-inducible factor (HIF) and RNApol2 binding in hypoxic cells. These analyses revealed that all classes of RNA are profoundly regulated by hypoxia and implicated HIF as a major direct regulator of both the non-coding and coding transcriptome, acting predominantly through release of pre-bound promoter-paused RNApol2. These findings indicate that the transcriptional response to hypoxia is substantially more extensive than previously considered.
Involved external institutions
United Kingdom (GB)How to cite
APA:
Choudhry, H., Schoedel, J., Oikonomopoulos, S., Camps, C., Grampp, S., Harris, A.L.,... Mole, D.R. (2014). Extensive regulation of the non-coding transcriptome by hypoxia: role of HIF in releasing paused RNApol2. EMBO Reports, 15(1), 70-6. https://doi.org/10.1002/embr.201337642
MLA:
Choudhry, Hani, et al. "Extensive regulation of the non-coding transcriptome by hypoxia: role of HIF in releasing paused RNApol2." EMBO Reports 15.1 (2014): 70-6.
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