Xyloglucan endotransglucosylase and cell wall extensibility

Journal article

Publication Details

Author(s): Miedes E, Zarra I, Hoson T, Herbers K, Sonnewald U, Lorences EP
Journal: Journal of Plant Physiology
Publication year: 2011
Volume: 168
Journal issue: 3
Pages range: 196-203
ISSN: 0176-1617


Transgenic tomato hypocotyls with altered levels of an XTH gene were used to study how XET activity could affect the hypocotyl growth and cell wall extensibility. Transgenic hypocotyls showed significant over-expression (line 13) or co-suppression (line 33) of the SIXTH1 in comparison with the wild type, with these results being correlated with the results on specific soluble XET activity, suggesting that SIXTH1 translates mainly for a soluble XET isoenzyme. A relationship between XET activity and cell wall extensibility was found, and the highest total extensibility was located in the apical hypocotyl segment of the over-expressing SIXTH1 line, where the XET-specific activity and hypocotyl growth were also highest compared with the wild line. Also, in the co-suppression SIXTH1 line, total extensibility values were lower than in the wild type line. The study of linkages between cell wall polysaccharides by FTIR showed that hypocotyls over-expressing SIXTH1 and having a higher XET-specific activity, were grouped away from the wild line, indicating that the linkages between pectins and between cellulose and xyloglucans might differ. These results suggested that the action of the increased XET activity in the transgenic line could be responsible for the cell wall structural changes, and therefore, alter the cell wall extensibility. On the other hand, results on xyloglucan oligosaccharides composition of the xyloglucan by MALDI TOF-MS showed no differences between lines, indicating that the xyloglucan structure was not affected by the XET action. These results provide evidences that XTHs from group I are involved mainly in the restructuring of the cell wall during growth and development, but they are not the limiting factor for plant growth. (C) 2010 Elsevier GmbH. All rights reserved.

FAU Authors / FAU Editors

Sonnewald, Uwe Prof. Dr.
Lehrstuhl für Biochemie

External institutions with authors

Osaka City University (OCU) / 大阪市立大学
Universidade de Santiago Compostela (USC)
University of València / Universitat de València

How to cite

Miedes, E., Zarra, I., Hoson, T., Herbers, K., Sonnewald, U., & Lorences, E.P. (2011). Xyloglucan endotransglucosylase and cell wall extensibility. Journal of Plant Physiology, 168(3), 196-203. https://dx.doi.org/10.1016/j.jplph.2010.06.029

Miedes, Eva, et al. "Xyloglucan endotransglucosylase and cell wall extensibility." Journal of Plant Physiology 168.3 (2011): 196-203.


Last updated on 2018-08-08 at 12:38