Signature of circulating microRNAs in osteoarthritis

Beyer C, Zampetaki A, Lin NY, Kleyer A, Perricone C, Iagnocco A, Distler A, Langley SR, Gelse K, Sesselmann S, Lorenzini R, Niemeier A, Swoboda B, Distler J, Santer P, Egger G, Willeit J, Mayr M, Schett G, Kiechl S (2015)

Publication Type: Journal article

Publication year: 2015

Journal

Annals of the Rheumatic Diseases BMJ Publishing Group

Book Volume: 74

Pages Range: e18

Journal Issue: 3

DOI: 10.1136/annrheumdis-2013-204698

Abstract

Osteoarthritis is the most common form of arthritis and a major socioeconomic burden. Our study is the first to explore the association between serum microRNA levels and the development of severe osteoarthritis of the knee and hip joint in the general population.We followed 816 Caucasian individuals from 1995 to 2010 and assessed joint arthroplasty as a definitive outcome of severe osteoarthritis of the knee and hip. After a microarray screen, we validated 12 microRNAs by real-time PCR in the entire cohort at baseline.In Cox regression analysis, three microRNAs were associated with severe knee and hip osteoarthritis. let-7e was a negative predictor for total joint arthroplasty with an adjusted HR of 0.75 (95% CI 0.58 to 0.96; p=0.021) when normalised to U6, and 0.76 (95% CI 0.6 to 0.97; p=0.026) after normalisation to the Ct average. miRNA-454 was inversely correlated with severe knee or hip osteoarthritis with an adjusted HR of 0.77 (95% CI 0.61 to 0.97; p=0.028) when normalised to U6. This correlation was lost when data were normalised to Ct average (p=0.118). Finally, miRNA-885-5p showed a trend towards a positive relationship with arthroplasty when normalised to U6 (HR 1.24; 95% CI 0.95 to 1.62; p=0.107) or to Ct average (HR 1.30; 95% CI 0.99 to 1.70; p=0.056).Our study is the first to identify differentially expressed circulating microRNAs in osteoarthritis patients necessitating arthroplasty in a large, population-based cohort. Among these microRNAs, let-7e emerged as potential predictor for severe knee or hip osteoarthritis.

Authors with CRIS profile

Christian Beyer Department of Medicine 3 – Rheumatology and Immunology Arnd Kleyer Department of Medicine 3 – Rheumatology and Immunology Stefan Sesselmann Departement of Orthopaedic Surgery Bernd Swoboda Professur für Orthopädische Rheumatologie Jörg Distler Professur für Molekulare Mechanismen der Organfibrose Georg Schett Lehrstuhl für Innere Medizin III

Involved external institutions

Universitätsklinikum Hamburg-Eppendorf (UKE) DE Germany (DE) Krankenhaus Bruneck / Ospedale di Brunico IT Italy (IT) King’s College London GB United Kingdom (GB) Università degli studi "La Sapienza" IT Italy (IT) Medizinische Universität Innsbruck AT Austria (AT)

How to cite

APA:

Beyer, C., Zampetaki, A., Lin, N.-Y., Kleyer, A., Perricone, C., Iagnocco, A.,... Kiechl, S. (2015). Signature of circulating microRNAs in osteoarthritis. Annals of the Rheumatic Diseases, 74(3), e18. https://dx.doi.org/10.1136/annrheumdis-2013-204698

MLA:

Beyer, Christian, et al. "Signature of circulating microRNAs in osteoarthritis." Annals of the Rheumatic Diseases 74.3 (2015): e18.

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