Transcriptome sequencing reveals maelstrom as a novel target gene of the terminal system in the red flour beetle Tribolium castaneum

Journal article


Publication Details

Author(s): Pridöhl F, Weißkopf M, Koniszewski N, Sulzmaier A, Uebe S, Ekici AB, Schoppmeier M
Journal: Development
Publication year: 2017
Volume: 144
Journal issue: 7
Pages range: 1339-1349
ISSN: 0950-1991
eISSN: 1477-9129


Abstract


Terminal regions of the Drosophila embryo are patterned by the localized activation of the Torso-RTK pathway, which promotes the downregulation of Capicua. In the short-germ beetle Tribolium, the function of the terminal system appears to be rather different, as the pathway promotes axis elongation and, in addition, is required for patterning the extra-embryonic serosa at the anterior. Here, we show that Torso signalling also induces gene expression by relieving Capicua-mediated repression in Tribolium Given that the majority of Torso target genes remain to be identified, we established a differential gene-expression screen. A subset of 50 putative terminal target genes was screened for functions in early embryonic patterning. Of those, 13 genes show early terminal expression domains and also phenotypes were related to terminal patterning. Among others, we found the PIWI-interacting RNA factor Maelstrom to be crucial for early embryonic polarization. Tc-mael is required for proper serosal size regulation and head morphogenesis. Moreover, Tc-mael promotes growth-zone formation and axis elongation. Our results suggest that posterior patterning by Torso may be realized through Maelstrom-dependent activation of posterior Wnt domains.



FAU Authors / FAU Editors

Ekici, Arif Bülent Dr. rer. nat.
Humangenetisches Institut
Pridöhl, Fabian
Lehrstuhl für Entwicklungsbiologie
Schoppmeier, Michael PD Dr.
Lehrstuhl für Entwicklungsbiologie
Sulzmaier, Andreas
Lehrstuhl für Entwicklungsbiologie
Uebe, Steffen Dr. rer. nat.
Humangenetisches Institut
Weißkopf, Matthias
Lehrstuhl für Entwicklungsbiologie


How to cite

APA:
Pridöhl, F., Weißkopf, M., Koniszewski, N., Sulzmaier, A., Uebe, S., Ekici, A.B., & Schoppmeier, M. (2017). Transcriptome sequencing reveals maelstrom as a novel target gene of the terminal system in the red flour beetle Tribolium castaneum. Development, 144(7), 1339-1349. https://dx.doi.org/10.1242/dev.136853

MLA:
Pridöhl, Fabian, et al. "Transcriptome sequencing reveals maelstrom as a novel target gene of the terminal system in the red flour beetle Tribolium castaneum." Development 144.7 (2017): 1339-1349.

BibTeX: 

Last updated on 2018-10-10 at 02:17