Evaluation of a 345 nm Femtosecond Laser for Corneal Surgery with Respect to Intraocular Radiation Hazard

Menzel-Severing J, Petsch C, Tourtas T, Polisetti N, Klenke J, Skerl K, Wuellner C, Donitzky C, Kruse F, Kremers J, Hammer C (2015)

Publication Type: Journal article

Publication year: 2015

Journal

PLoS ONE Public Library of Science

Book Volume: 10

Pages Range: e0137638

Journal Issue: 9

DOI: 10.1371/journal.pone.0137638

Abstract

We report our findings from a preclinical safety study designed to assess potential side effects of corneal ultraviolet femtosecond laser treatment on lens and retina.Refractive lenticules (-5 dpt) with a diameter of 6 mm were created in the right cornea of eight Dutch Belted rabbits. Radiant exposure was 0.5 J/cm² in two animals and 18 J/cm² in six animals. The presence of lens opacities was assessed prior to and up to six months following laser application using Scheimpflug images (Pentacam, Oculus) and backscatter analysis (Opacity Lensmeter 702, Interzeag). Ganzfeld flash and flicker electroretinogram (ERG) recordings were obtained from both eyes prior to and up to six weeks following laser application. At the study endpoint, retinas were examined by light microscopy.Independent of energy dose applied, no cataract formation could be observed clinically or with either of the two objective methods used. No changes in ERG recordings over time and no difference between treated and untreated eye were detected. Histologically, retinal morphology was preserved and retinal pigment epithelium as well as photoreceptor inner and outer segments appeared undamaged. Quantitative digital image analysis did not reveal cell loss in inner or outer nuclear layers.Our analysis confirms theoretical considerations suggesting that ultraviolet femtosecond laser treatment of the cornea is safe for intraocular tissues. Transmitted light including stray light induces no photochemical effects in lens or retina at energy levels much higher than required for the clinical purpose. These conclusions cannot be applied to eyes with pre-existing retinal damage, as these may be more vulnerable to light.

Authors with CRIS profile

Johannes Menzel-Severing Department of Ophthalmology Friedrich Kruse Lehrstuhl für Augenheilkunde Jan Kremers Professur für Experimentelle Augenheilkunde Christian Hammer Lehrstuhl für Funktionelle und Klinische Anatomie

Involved external institutions

WaveLight GmbH DE Germany (DE)

How to cite

APA:

Menzel-Severing, J., Petsch, C., Tourtas, T., Polisetti, N., Klenke, J., Skerl, K.,... Hammer, C. (2015). Evaluation of a 345 nm Femtosecond Laser for Corneal Surgery with Respect to Intraocular Radiation Hazard. PLoS ONE, 10(9), e0137638. https://dx.doi.org/10.1371/journal.pone.0137638

MLA:

Menzel-Severing, Johannes, et al. "Evaluation of a 345 nm Femtosecond Laser for Corneal Surgery with Respect to Intraocular Radiation Hazard." PLoS ONE 10.9 (2015): e0137638.

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