Autologous serum improves bone formation in a primary stable silica-embedded nanohydroxyapatite bone substitute in combination with mesenchymal stem cells and rhBMP-2 in the sheep model

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Details zur Publikation

Autorinnen und Autoren: Boos A, Weigand A, Deschler G, Gerber T, Arkudas A, Kneser U, Horch RE, Beier J
Zeitschrift: International Journal of Nanomedicine
Jahr der Veröffentlichung: 2014
Band: 9
Seitenbereich: 5317-39
ISSN: 1176-9114
eISSN: 1178-2013


Abstract


New therapeutic strategies are required for critical size bone defects, because the gold standard of transplanting autologous bone from an unharmed area of the body often leads to several severe side effects and disadvantages for the patient. For years, tissue engineering approaches have been seeking a stable, axially vascularized transplantable bone replacement suitable for transplantation into the recipient bed with pre-existing insufficient conditions. For this reason, the arteriovenous loop model was developed and various bone substitutes have been vascularized. However, it has not been possible thus far to engineer a primary stable and axially vascularized transplantable bone substitute. For that purpose, a primary stable silica-embedded nanohydroxyapatite (HA) bone substitute in combination with blood, bone marrow, expanded, or directly retransplanted mesenchymal stem cells, recombinant human bone morphogenetic protein 2 (rhBMP-2), and different carrier materials (fibrin, cell culture medium, autologous serum) was tested subcutaneously for 4 or 12 weeks in the sheep model. Autologous serum lead to an early matrix change during degradation of the bone substitute and formation of new bone tissue. The best results were achieved in the group combining mesenchymal stem cells expanded with 60 ?g/mL rhBMP-2 in autologous serum. Better ingrowth of fibrovascular tissue could be detected in the autologous serum group compared with the control (fibrin). Osteoclastic activity indicating an active bone remodeling process was observed after 4 weeks, particularly in the group with autologous serum and after 12 weeks in every experimental group. This study clearly demonstrates the positive effects of autologous serum in combination with mesenchymal stem cells and rhBMP-2 on bone formation in a primary stable silica-embedded nano-HA bone grafting material in the sheep model. In further experiments, the results will be transferred to the sheep arteriovenous loop model in order to engineer an axially vascularized primary stable bone replacement in clinically relevant size for free transplantation.



FAU-Autorinnen und Autoren / FAU-Herausgeberinnen und Herausgeber

Arkudas, Andreas Prof. Dr.
Medizinische Fakultät
Boos, Anja Dr.
Plastisch- und Handchirurgische Klinik
Deschler, Gloria
Medizinische Fakultät
Horch, Raymund E. Prof. Dr.
Professur für Plastische Chirurgie und Handchirurgie
Kengelbach-Weigand, Annika Dr.
Plastisch- und Handchirurgische Klinik


Einrichtungen weiterer Autorinnen und Autoren

Universität Rostock


Zitierweisen

APA:
Boos, A., Weigand, A., Deschler, G., Gerber, T., Arkudas, A., Kneser, U.,... Beier, J. (2014). Autologous serum improves bone formation in a primary stable silica-embedded nanohydroxyapatite bone substitute in combination with mesenchymal stem cells and rhBMP-2 in the sheep model. International Journal of Nanomedicine, 9, 5317-39. https://dx.doi.org/10.2147/IJN.S66867

MLA:
Boos, Anja, et al. "Autologous serum improves bone formation in a primary stable silica-embedded nanohydroxyapatite bone substitute in combination with mesenchymal stem cells and rhBMP-2 in the sheep model." International Journal of Nanomedicine 9 (2014): 5317-39.

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