S100A1 decreases calcium spark frequency and alters their spatial characteristics in permeabilized adult ventricular cardiomyocytes.

Beitrag in einer Fachzeitschrift
(Originalarbeit)


Details zur Publikation

Autorinnen und Autoren: Völkers M, Loughrey CM, MacQuaide N, Remppis BA, DeGeorge BR, von Wegner F, Friedrich O, Fink R, Koch WJ, Smith GL, Most P
Zeitschrift: Cell Calcium
Verlag: Elsevier
Jahr der Veröffentlichung: 2007
Band: 41
Heftnummer: 2
Seitenbereich: 135-43
ISSN: 0143-4160
Sprache: Englisch


Abstract


S100A1, a Ca2+-sensor protein of the EF-hand type, exerts positive inotropic effects in the heart via enhanced cardiac ryanodine receptor (RyR2) activity. Here we report that S100A1 protein (0.1microM) interacts with the RyR2 in resting permeabilized cardiomyocytes at free Ca2+-levels comparable to diastolic Ca2+-concentrations ( approximately 150nM). Alterations of RyR2 function due to S100A1 binding was assessed via analysis of Ca2+-spark characteristics. Ca2+-spark frequency, amplitude and duration were all reduced upon perfusion with 0.1microM S100A1 protein by 38%, 14% and 18%, respectively. Most likely, these effects were conveyed through the S100A1 C-terminus (S100A1-ct; amino acids 75-94) as the corresponding S100A1-ct peptide (0.1microM) inhibited S100A1 protein binding to the RyR2 and similarly attenuated frequency, amplitude and duration of Ca2+-sparks by 52%, 8% and 26%, respectively. Accordingly, the sarcoplasmic reticulum (SR) Ca2+-content was slightly increased but the stoichiometry of other accessory RyR2 modulators (sorcin/FKBP12.6) remained unaltered by S100A1. Hence, we propose S100A1 as a novel inhibitory modulator of RyR2 function at diastolic Ca2+-concentrations in rabbit ventricular cardiomyocytes.



FAU-Autorinnen und Autoren / FAU-Herausgeberinnen und Herausgeber

Friedrich, Oliver Prof. Dr. Dr.
Lehrstuhl für Medizinische Biotechnologie


Einrichtungen weiterer Autorinnen und Autoren

Goethe-Universität Frankfurt am Main
Ruprecht-Karls-Universität Heidelberg
Temple University
Thomas Jefferson University
Universitätsklinikum Heidelberg
University of Glasgow


Zitierweisen

APA:
Völkers, M., Loughrey, C.M., MacQuaide, N., Remppis, B.A., DeGeorge, B.R., von Wegner, F.,... Most, P. (2007). S100A1 decreases calcium spark frequency and alters their spatial characteristics in permeabilized adult ventricular cardiomyocytes. Cell Calcium, 41(2), 135-43. https://dx.doi.org/10.1016/j.ceca.2006.06.001

MLA:
Völkers, Mirko, et al. "S100A1 decreases calcium spark frequency and alters their spatial characteristics in permeabilized adult ventricular cardiomyocytes." Cell Calcium 41.2 (2007): 135-43.

BibTeX: 

Zuletzt aktualisiert 2018-17-10 um 06:20