STP10 encodes a high-affinity monosaccharide transporter and is induced under low-glucose conditions in pollen tubes of Arabidopsis.

Beitrag in einer Fachzeitschrift
(Originalarbeit)


Details zur Publikation

Autorinnen und Autoren: Rottmann T, Zierer W, Subert C, Sauer N, Stadler R
Zeitschrift: Journal of Experimental Botany
Jahr der Veröffentlichung: 2016
Band: 67
Heftnummer: 8
Seitenbereich: 2387-99
ISSN: 1460-2431
Sprache: Englisch


Abstract


Pollen tubes are fast growing, photosynthetically inactive cells. Their energy demand is covered by specific transport proteins in the plasma membrane that mediate the uptake of sugars. Here we report on the functional characterization of AtSTP10, a previously uncharacterized member of the SUGAR TRANSPORT PROTEIN family. Heterologous expression of STP10 cDNA in yeast revealed that the encoded protein catalyses the high-affinity uptake of glucose, galactose and mannose. The transporter is sensitive to uncouplers of transmembrane proton gradients, indicating that the protein acts as a hexose-H(+)symporter. Analyses of STP10 mRNA and STP10 promoter-reporter gene studies revealed a sink-specific expression pattern of STP10 in primordia of lateral roots and in pollen tubes. This restriction to sink organs is mediated by intragenic regions of STP10 qPCR analyses with cDNA of in vitro grown pollen tubes showed that STP10 expression was down-regulated in the presence of 50mM glucose. However, in pollen tubes of glucose-insensitive plants, which lack the glucose sensor hexokinase1 (HXK1), no glucose-induced down-regulation of STP10 expression was detected. A stp10T-DNA insertion line developed normally, which may point towards functional redundancy. The data presented in this paper indicate that a high-affinity glucose uptake system is induced in growing pollen tubes under low glucose conditions and that this regulation may occur through the hexokinase pathway.



FAU-Autorinnen und Autoren / FAU-Herausgeberinnen und Herausgeber

Rottmann, Theresa
Lehrstuhl für Molekulare Pflanzenphysiologie
Sauer, Norbert Prof. Dr.
Lehrstuhl für Molekulare Pflanzenphysiologie
Stadler, Ruth PD Dr.
Lehrstuhl für Molekulare Pflanzenphysiologie
Zierer, Wolfgang Dr.
Lehrstuhl für Molekulare Pflanzenphysiologie


Zitierweisen

APA:
Rottmann, T., Zierer, W., Subert, C., Sauer, N., & Stadler, R. (2016). STP10 encodes a high-affinity monosaccharide transporter and is induced under low-glucose conditions in pollen tubes of Arabidopsis. Journal of Experimental Botany, 67(8), 2387-99. https://dx.doi.org/10.1093/jxb/erw048

MLA:
Rottmann, Theresa, et al. "STP10 encodes a high-affinity monosaccharide transporter and is induced under low-glucose conditions in pollen tubes of Arabidopsis." Journal of Experimental Botany 67.8 (2016): 2387-99.

BibTeX: 

Zuletzt aktualisiert 2018-17-10 um 05:20