An N-terminal dileucine motif directs two-pore channels to the tonoplast of plant cells.

Larisch N, Schulze C, Galione A, Dietrich P (2012)

Publication Language: English

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2012

Journal

Traffic (Copenhagen, Denmark)

Book Volume: 13

Pages Range: 1012-22

Journal Issue: 7

DOI: 10.1111/j.1600-0854.2012.01366.x

Open Access Link: http://onlinelibrary.wiley.com/doi/10.1111/j.1600-0854.2012.01366.x/abstract?systemMessage=Wiley+Online+Library+will+be+unavailable+on+Saturday+25th+March+from+07%3A00+GMT+%2F+03%3A00+EDT+%2F+15%3A00+SGT+for+4+hours+for+essential+maintenance.++Apolog

Abstract

Two-pore channels (TPCs) constitute a family of endolysosomal cation channels with functions in Ca²⁺ signaling. We used a mutational analysis to investigate the role of channel domains for the trafficking of the Arabidopsis TPC1 to the tonoplast, a process that is generally not well understood in plants. The results show that the soluble C-terminus was not essential for targeting but for channel function, while further C-terminal truncations of two or more transmembrane domains impaired protein trafficking. An N-terminal dileucine motif (EDPLI) proved to be critical for vacuolar targeting of TPC1, which was independent of the adaptor protein AP-3. Deletion or mutation of this sorting motif, which is conserved among TPCs caused redirection of the protein transport to the plasma membrane. An N-terminal region with a predicted α-helical structure was shown to support efficient vacuolar trafficking and was essential for TPC1 function. Similar to their localization in mammalian endosomes and lysosomes, MmTPC1 and MmTPC2 were targeted to small organelles and the membrane of the lytic vacuole, respectively, when expressed in plant cells. These results shed new light on the largely uncharacterized sorting signals of plant tonoplast proteins and reveal similarities between the targeting machinery of plants and mammals.

Authors with CRIS profile

Christina Schulze Professur für Zellbiologie der Pflanzen Petra Dietrich Professur für Zellbiologie der Pflanzen

Involved external institutions

University of Oxford GB United Kingdom (GB)

How to cite

APA:

Larisch, N., Schulze, C., Galione, A., & Dietrich, P. (2012). An N-terminal dileucine motif directs two-pore channels to the tonoplast of plant cells. Traffic (Copenhagen, Denmark), 13(7), 1012-22. https://dx.doi.org/10.1111/j.1600-0854.2012.01366.x

MLA:

Larisch, Nina, et al. "An N-terminal dileucine motif directs two-pore channels to the tonoplast of plant cells." Traffic (Copenhagen, Denmark) 13.7 (2012): 1012-22.

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