Study of the anti-inflammatory effects of low-dose radiation: The contribution of biphasic regulation of the antioxidative system in endothelial cells

Large M, Hehlgans S, Reichert S, Gaipl U, Fournier C, Rödel C, Weiss C, Rödel F (2015)

Publication Type: Journal article

Publication year: 2015

Journal

Strahlentherapie und Onkologie Urban & Vogel

Publisher: Springer Verlag (Germany)

Book Volume: 191

Pages Range: 742-9

Journal Issue: 9

DOI: 10.1007/s00066-015-0848-9

Abstract

We examined (a) the expression of the antioxidative factor glutathione peroxidase (GPx) and the transcription factor nuclear factor E2-related factor 2 (Nrf2) following low-dose X-irradiation in endothelial cells (ECs) and (b) the impact of reactive oxygen species (ROS) and Nrf2 on functional properties of ECs to gain further knowledge about the anti-inflammatory mode of action of low doses of ionizing radiation.EA.hy926 ECs and primary human dermal microvascular ECs (HMVEC) were stimulated by tumor necrosis factor-? (TNF-?, 20 ng/ml) 4 h before irradiation with single doses ranging from 0.3 to 3 Gy. The expression and activity of GPx and Nrf2 were analyzed by flow cytometry, colorimetric assays, and real-time PCR. The impact of ROS and Nrf2 on peripheral blood mononuclear cell (PBMC) adhesion was assayed in the presence of the ROS scavenger N-acetyl-L-cysteine (NAC) and Nrf2 activator AI-1.Following a low-dose exposure, we observed in EA.hy926 EC and HMVECs a discontinuous expression and enzymatic activity of GPx concomitant with a lowered expression and DNA binding activity of Nrf2 that was most pronounced at a dose of 0.5 Gy. Scavenging of ROS by NAC and activation of Nrf2 by AI-1 significantly diminished a lowered adhesion of PBMC to EC at a dose of 0.5 Gy.Low-dose irradiation resulted in a nonlinear expression and activity of major compounds of the antioxidative system that might contribute to anti-inflammatory effects in stimulated ECs.

Authors with CRIS profile

Udo Gaipl Department of Radiation Oncology

Involved external institutions

Goethe-Universität Frankfurt am Main DE Germany (DE) GSI Helmholtzzentrum für Schwerionenforschung GmbH DE Germany (DE)

How to cite

APA:

Large, M., Hehlgans, S., Reichert, S., Gaipl, U., Fournier, C., Rödel, C.,... Rödel, F. (2015). Study of the anti-inflammatory effects of low-dose radiation: The contribution of biphasic regulation of the antioxidative system in endothelial cells. Strahlentherapie und Onkologie, 191(9), 742-9. https://dx.doi.org/10.1007/s00066-015-0848-9

MLA:

Large, Martin, et al. "Study of the anti-inflammatory effects of low-dose radiation: The contribution of biphasic regulation of the antioxidative system in endothelial cells." Strahlentherapie und Onkologie 191.9 (2015): 742-9.

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