KAT1 is not essential for stomatal opening.

Szyroki A, Ivashikina N, Dietrich P, Roelfsema MRG, Ache P, Reintanz B, Deeken R, Godde M, Felle H, Steinmeyer R, Palme K, Hedrich R (2001)

Publication Language: English

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2001

Journal

Proceedings of the National Academy of Sciences of the United States of America National Academy of Sciences

Book Volume: 98

Pages Range: 2917-21

Journal Issue: 5

DOI: 10.1073/pnas.051616698

Abstract

It is generally accepted that K(+) uptake into guard cells via inward-rectifying K(+) channels is required for stomatal opening. To test whether the guard cell K(+) channel KAT1 is essential for stomatal opening, a knockout mutant, KAT1En-1, was isolated from an En-1 mutagenized Arabidopsis thaliana population. Stomatal action and K(+) uptake, however, were not impaired in KAT1-deficient plants. Reverse transcription-PCR experiments with isolated guard cell protoplasts showed that in addition to KAT1, the K(+) channels AKT1, AKT2/3, AtKC1, and KAT2 were expressed in this cell type. In impalement measurements, intact guard cells exhibited inward-rectifying K(+) currents across the plasma membrane of both wild-type and KAT1En-1 plants. This study demonstrates that multiple K(+) channel transcripts exist in guard cells and that KAT1 is not essential for stomatal action.

Authors with CRIS profile

Petra Dietrich Professur für Zellbiologie der Pflanzen

Involved external institutions

Max-Delbrück-Centrum für Molekulare Medizin / Max Delbrück Center for Molecular Medicine (MDC) Berlin-Buch DE Germany (DE) Julius-Maximilians-Universität Würzburg DE Germany (DE) Justus-Liebig-Universität Gießen DE Germany (DE)

How to cite

APA:

Szyroki, A., Ivashikina, N., Dietrich, P., Roelfsema, M.R.G., Ache, P., Reintanz, B.,... Hedrich, R. (2001). KAT1 is not essential for stomatal opening. Proceedings of the National Academy of Sciences of the United States of America, 98(5), 2917-21. https://doi.org/10.1073/pnas.051616698

MLA:

Szyroki, Alexander, et al. "KAT1 is not essential for stomatal opening." Proceedings of the National Academy of Sciences of the United States of America 98.5 (2001): 2917-21.

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