Ex Vivo Apoptosis in CD8+ Lymphocytes Predicts Rectal Cancer Patient Outcome

Winkler S, Hoppe P, Haderlein M, Hecht M, Fietkau R, Distel L (2016)

Publication Type: Journal article

Publication year: 2016

Journal

Gastroenterology Research and Practice Hindawi Publishing Corporation

Book Volume: 2016

Pages Range: 5076542

DOI: 10.1155/2016/5076542

Abstract

Background. Apoptotic rates in peripheral blood lymphocytes can predict radiation induced normal tissue toxicity. We studied whether apoptosis in lymphocytes has a prognostic value for therapy outcome. Methods. Lymphocytes of 87 rectal cancer patients were ex vivo irradiated with 2 Gy, 8 Gy, or a combination of 2 Gy ionizing radiation and Oxaliplatin. Cells were stained with Annexin V and 7-Aminoactinomycin D and apoptotic and necrotic rates were analyzed by multicolor flow cytometry. Results. After treatment, apoptotic and necrotic rates in CD8+ cells are consistently higher than in CD4+ cells, with lower corresponding necrotic rates. Apoptotic and necrotic rates of CD4+ cells and CD8+ cells correlated well within the 2 Gy, 8 Gy, and 2 Gy and Oxaliplatin arrangements (p <= 0.009). High apoptotic CD8+ rates after 2 Gy, 8 Gy, and 2 Gy + Oxaliplatin treatment were prognostically favorable for metastasis-free survival (p = 0.009, p = 0.038, and p = 0.009) and disease-free survival (p = 0.013, p = 0.098, and p = 0.013). Conclusions. Ex vivo CD8+ apoptotic rates are able to predict the patient outcome in regard to metastasis-free or disease-free survival. Patients with higher CD8+ apoptotic rates in the peripheral blood have a more favorable prognosis. In addition to the prediction of late-toxicity by utilization of CD4+ apoptotic rates, the therapy outcome can be predicted by CD8+ apoptotic rates.

Authors with CRIS profile

Sebastian Winkler Medizinische Fakultät Philipp Hoppe Medizinische Fakultät Marlen Haderlein Department of Radiation Oncology Markus Hecht Department of Radiation Oncology Rainer Fietkau Lehrstuhl für Strahlentherapie Luitpold Distel Department of Radiation Oncology

How to cite

APA:

Winkler, S., Hoppe, P., Haderlein, M., Hecht, M., Fietkau, R., & Distel, L. (2016). Ex Vivo Apoptosis in CD8+ Lymphocytes Predicts Rectal Cancer Patient Outcome. Gastroenterology Research and Practice, 2016, 5076542. https://dx.doi.org/10.1155/2016/5076542

MLA:

Winkler, Sebastian, et al. "Ex Vivo Apoptosis in CD8+ Lymphocytes Predicts Rectal Cancer Patient Outcome." Gastroenterology Research and Practice 2016 (2016): 5076542.

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