The pro-angiogenic properties of multi-functional bioactive glass composite scaffolds
Gerhardt LC, Widdows KL, Erol MM, Burch CW, Sanz-Herrera JA, Ochoa I, Stämpfli R, Roqan IS, Gabe S, Ansari T, Boccaccini AR (2011)
Publication Type: Journal article
Publication year: 2011
Journal
Publisher: Elsevier
Pages Range: 4096-4108
Journal Issue: 32
DOI: 10.1016/j.biomaterials.2011.02.032
Abstract
The angiogenic properties of micron-sized (m-BG) and nano-sized (n-BG) bioactive glass (BG) filled poly(D,L lactide) (PDLLA) composites were investigated. On the basis of cell culture work investigating the secretion of vascular endothelial growth factor (VEGF) by human fibroblasts in contact with composite films (0, 5, 10, 20 wt %), porous 3D composite scaffolds, optimised with respect to the BG filler content capable of inducing angiogenic response, were produced. The in vivo vascularisation of the scaffolds was studied in a rat animal model and quantified using stereological analyses. The prepared scaffolds had high porosities (81-93%), permeability (k = 5.4-8.6 × 10-9 m2) and compressive strength values (0.4-1.6 MPa) all in the range of trabecular bone. On composite films containing 20 wt % m-BG or n-BG, human fibroblasts produced 5 times higher VEGF than on pure PDLLA films. After 8 weeks of implantation, m-BG and n-BG containing scaffolds were well-infiltrated with newly formed tissue and demonstrated higher vascularisation and percentage blood vessel to tissue (11.6-15.1%) than PDLLA scaffolds (8.5%). This work thus shows potential for the regeneration of hard-soft tissue defects and increased bone formation arising from enhanced vascularisation of the construct. © 2011 Elsevier Ltd.
Authors with CRIS profile
Involved external institutions
Imperial College London / The Imperial College of Science, Technology and Medicine
United Kingdom (GB)
Istanbul Technical University / İstanbul Teknik Üniversitesi (İTÜ)
Turkey (TR)
University of Seville / Universidad de Sevilla
Spain (ES)
Universidad de Zaragoza
Spain (ES)
Eidgenössische Materialprüfungs- und Forschungsanstalt (Empa) / Swiss Federal Laboratories for Materials Science & Technology
Switzerland (CH)
King Abdullah University of Science and Technology (KAUST) / جامعة الملك عبد الله للعلوم و التقنية
Saudi Arabia (SA)
St. Mark's Hospital
United Kingdom (GB)
United Kingdom (GB)
Istanbul Technical University / İstanbul Teknik Üniversitesi (İTÜ)
Turkey (TR)
University of Seville / Universidad de Sevilla
Spain (ES)
Universidad de Zaragoza
Spain (ES)
Eidgenössische Materialprüfungs- und Forschungsanstalt (Empa) / Swiss Federal Laboratories for Materials Science & Technology
Switzerland (CH)
King Abdullah University of Science and Technology (KAUST) / جامعة الملك عبد الله للعلوم و التقنية
Saudi Arabia (SA)
St. Mark's Hospital
United Kingdom (GB)
How to cite
APA:
Gerhardt, L.-C., Widdows, K.L., Erol, M.M., Burch, C.W., Sanz-Herrera, J.A., Ochoa, I.,... Boccaccini, A.R. (2011). The pro-angiogenic properties of multi-functional bioactive glass composite scaffolds. Biomaterials, 32, 4096-4108. https://dx.doi.org/10.1016/j.biomaterials.2011.02.032
MLA:
Gerhardt, Lutz-Christian, et al. "The pro-angiogenic properties of multi-functional bioactive glass composite scaffolds." Biomaterials 32 (2011): 4096-4108.
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