The influence of donor factors on corneal organ culture contamination

Gruenert AK, Rosenbaum K, Geerling G, Fuchsluger T (2017)

Publication Type: Journal article

Publication year: 2017

Journal

Acta Ophthalmologica Wiley-Blackwell

DOI: 10.1111/aos.13402

Abstract

To evaluate the contamination rate and the corresponding spectrum of microbes and to identify donor risk factors for corneal organ culture contaminations.A total of 3306 organ-cultured donor corneas were included in the study. We performed a retrospective database analysis to evaluate donor factors such as gender, age, death-to-explantation interval (DEI), procurement site and cause of death and to determine their influence on donor cornea contaminations. Odds ratios (ORs) were calculated for each factor.The overall contamination rate was 7.8% (n = 259). Younger donor age (OR: 2.2, p = 0.003, chi-squared test), a DEI of more than 24 hr (OR: 1.6, p < 0.001), hospitalization prior to death (OR: 2.2, p < 0.001) and death caused by sepsis (OR: 2.7, p < 0.001) were associated with an increased risk of contamination, whereas donor gender did not have an effect on donor cornea contaminations. The most frequently isolated microbes were Enterococci (19%), Staphylococci (10.8%) and Candida (37.4%).This study helps to estimate the contamination risk of a cultured cornea based on specific donor factors. However, donors with risk factors should not be generally excluded from cornea donation. Further studies including antibiograms might clarify whether a change in the antibiotic composition of the culture medium would be useful to deal with the increasing number of multi-resistant microbes.

Authors with CRIS profile

Thomas Fuchsluger Professur für Augenheilkunde (Cornea/vorderer Augenabschnitt)

Involved external institutions

Heinrich-Heine-Universität Düsseldorf DE Germany (DE)

How to cite

APA:

Gruenert, A.K., Rosenbaum, K., Geerling, G., & Fuchsluger, T. (2017). The influence of donor factors on corneal organ culture contamination. Acta Ophthalmologica. https://doi.org/10.1111/aos.13402

MLA:

Gruenert, Anja K., et al. "The influence of donor factors on corneal organ culture contamination." Acta Ophthalmologica (2017).

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