High frequency of autoantibody-secreting cells and long-lived plasma cells within inflamed kidneys of NZB/W F1 lupus mice

Starke C, Frey S, Wellmann U, Urbonaviciute V, Herrmann M, Amann KU, Schett G, Winkler T, Voll RE (2011)

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2011

Journal

European Journal of Immunology Wiley-VCH Verlag

Publisher: WILEY-BLACKWELL

Book Volume: 41

Pages Range: 2107-2112

Journal Issue: 7

DOI: 10.1002/eji.201041315

Abstract

Autoantibodies to double-stranded (ds) DNA represent a serological hallmark of systemic lupus erythematosus (SLE) and may critically contribute to the pathogenesis of lupus nephritis. Self-reactive antibodies might be partially produced by long-lived plasma cells (PCs), which mainly reside within the bone marrow and spleen. In contrast to short-lived PCs, long-lived PCs are extremely resistant to therapy and may sustain refractory disease courses. Recently, antibody-secreting cells were found within the inflamed kidneys of New Zealand black/white (NZB/W) F1 lupus mice as well as of patients with SLE. To analyze the longevity of the IgG-producing cells present in nephritic kidneys of NZB/W F1 mice we performed in vivo BrdU-labeling. We identified a higher frequency of long-lived than short-lived renal PCs, indicating that survival niches for long-lived PCs also exist within inflamed kidneys. Using ELISPOT assays, we found that on average 31% of renal IgG-producing cells reacted with dsDNA and 24% with nucleolin. Moreover, the frequencies of IgG-secreting cells specific for the autoantigens dsDNA and nucleolin were higher in the kidneys compared with those in the spleen and bone marrow.

Authors with CRIS profile

Silke Frey Department of Medicine 3 – Rheumatology and Immunology Martin Herrmann Department of Medicine 3 – Rheumatology and Immunology Kerstin Ute Amann Department of Nephropathology Georg Schett Lehrstuhl für Innere Medizin III Thomas Winkler Professur für Genetik

Involved external institutions

Universitätsklinikum Freiburg DE Germany (DE)

How to cite

APA:

Starke, C., Frey, S., Wellmann, U., Urbonaviciute, V., Herrmann, M., Amann, K.U.,... Voll, R.E. (2011). High frequency of autoantibody-secreting cells and long-lived plasma cells within inflamed kidneys of NZB/W F1 lupus mice. European Journal of Immunology, 41(7), 2107-2112. https://doi.org/10.1002/eji.201041315

MLA:

Starke, Charlotte, et al. "High frequency of autoantibody-secreting cells and long-lived plasma cells within inflamed kidneys of NZB/W F1 lupus mice." European Journal of Immunology 41.7 (2011): 2107-2112.

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