Electroporation of siRNA into Mouse Bone Marrow-Derived Macrophages and Dendritic Cells
Siegert I, Schatz V, Prechtel AT, Steinkasserer A, Bogdan C, Jantsch J (2014)
Publication Type: Journal article
Publication year: 2014
Journal
Publisher: Humana Press, Inc.
Book Volume: 1121
Pages Range: 111-9
DOI: 10.1007/978-1-4614-9632-8_9
Abstract
Dendritic cells (DC) and macrophages (M?) play a pivotal role in antimicrobial defense, in the regulation of immune responses, and in maintaining tissue homeostasis. The analysis of DC and M? function relies on primary cells albeit these cells are known to be difficult to transfect. This makes the use of small interfering RNA (siRNA) for targeted manipulation of gene expression by RNA interference difficult. In the following chapter, we provide a detailed protocol for the successful transfer of siRNA via electroporation into a defined population of mouse bone marrow-derived M? or DC that does not cause toxicity to the myeloid cells or nonspecific alterations of their biological functions. Factors that influence the transfection and knockdown rate will be highlighted.
Authors with CRIS profile
Isabel Siegert
Lehrstuhl für Genetik
Alexander Steinkasserer
Professur für Experimentelle Dermatologie
Christian Bogdan
Lehrstuhl für Mikrobiologie und Infektionsimmunologie
Involved external institutions
Germany (DE)How to cite
APA:
Siegert, I., Schatz, V., Prechtel, A.T., Steinkasserer, A., Bogdan, C., & Jantsch, J. (2014). Electroporation of siRNA into Mouse Bone Marrow-Derived Macrophages and Dendritic Cells. Methods in Molecular Biology, 1121, 111-9. https://dx.doi.org/10.1007/978-1-4614-9632-8_9
MLA:
Siegert, Isabel, et al. "Electroporation of siRNA into Mouse Bone Marrow-Derived Macrophages and Dendritic Cells." Methods in Molecular Biology 1121 (2014): 111-9.
BibTeX: Download