Immunohistochemical and molecular characterizations in urothelial carcinoma of bladder in patients less than 45 years

Weyerer V, Schneckenpointner R, Filbeck T, Burger M, Hofstaedter F, Wild PJ, Fine SW, Humphrey PA, Dehner LP, Amin MB, Rueschoff J, Boltze C, Tannapfel A, Zwarthoff E, Lopez-Beltran A, Montironi R, Langner C, Stoehr R, Hartmann A, Giedl J (2017)

Publication Type: Journal article

Publication year: 2017

Journal

Journal of Cancer Ivyspring International Publisher

Book Volume: 8

Pages Range: 323-331

Journal Issue: 3

DOI: 10.7150/jca.17482

Abstract

Bladder tumours in early-onset patients are rare and seem to exhibit unique clinicopathological features. Only few studies have investigated somatic alterations in this specific age of onset group and evidence is accumulating of a distinct molecular behaviour of early-onset bladder tumours. We collected the largest cohort of early-onset tumours of patients 45 years old or younger and aimed to test genomic alterations typically found in bladder cancer. Tumours of 118 early-onset patients were compared with a consecutive group of 113 cases. Immunohistochemistry of TP53, CK20 and Ki-67 was carried out. Molecular analysis was conducted to test for loss of heterozygosity of chromosome 9 and 17, as well as TP53 and FGFR3 mutations. Fisher´s exact and chi-squared test were appropriately used. No differences in grade/stage characteristics were observed. Overexpressed TP53 was differentially distributed between the two groups. TP53 nuclear accumulation was significantly more frequent in early-onset papillomas, PUNLMPs and pTa low-grade tumours compared to the consecutive cohort (p=0.005). Moreover, chromosome 9 deletions (29.5% vs. 44.6%) and FGFR3 mutations (34.5% vs. 63.7%) were less often detected in early-onset patients (p=0.05 and p<0.0001). By comparing the largest cohort of early-onset bladder cancer patients with an unselected group, we demonstrated that the typical molecular features are not independent of age at diagnosis. Our study supports the hypothesis of a distinct biological behaviour in early-onset tumours.

Authors with CRIS profile

Arndt Hartmann Lehrstuhl für Allgemeine Pathologie und Pathologische Anatomie

Involved external institutions

Universitätsklinikum Regensburg DE Germany (DE) Universität Regensburg DE Germany (DE) Universitätsspital Zürich (USZ) CH Switzerland (CH) Memorial Sloan Kettering Cancer Center US United States (USA) (US) Yale University US United States (USA) (US) Washington University US United States (USA) (US) Cedars-Sinai Medical Center US United States (USA) (US) Ruhr-Universität Bochum (RUB) DE Germany (DE) SRH Wald-Klinikum Gera DE Germany (DE) Erasmus University Medical Center (MC) NL Netherlands (NL) University of Cordoba / Universidad de Córdoba (UCO) ES Spain (ES) Università Politecnica delle Marche (UNIVPM) / Marche Polytechnic University IT Italy (IT) Medizinische Universität Graz AT Austria (AT) Institut für Pathologie Nordhessen DE Germany (DE)

How to cite

APA:

Weyerer, V., Schneckenpointner, R., Filbeck, T., Burger, M., Hofstaedter, F., Wild, P.J.,... Giedl, J. (2017). Immunohistochemical and molecular characterizations in urothelial carcinoma of bladder in patients less than 45 years. Journal of Cancer, 8(3), 323-331. https://dx.doi.org/10.7150/jca.17482

MLA:

Weyerer, Veronika, et al. "Immunohistochemical and molecular characterizations in urothelial carcinoma of bladder in patients less than 45 years." Journal of Cancer 8.3 (2017): 323-331.

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