Filipovic M, Miljkovic J, Allgäuer A, Chaurio Gonzalez RA, Shubina T, Herrmann M, Ivanovic-Burmazovic I (2012)
Publication Type: Journal article, Original article
Publication year: 2012
Original Authors: Filipovic M., Miljkovic J., Allgäuer A., Chaurio R., Shubina T., Herrmann M., Ivanovic-Burmazovic I.
Publisher: Portland Press
Book Volume: 441
Pages Range: 609-621
Journal Issue: 2
DOI: 10.1042/BJ20111389
The reaction of hydrogen sulfide (H S) with peroxynitrite (a key mediator in numerous pathological states) was studied in vitro and in different cellularmodels. The results show that H S can scavenge peroxynitrite with a corresponding second order rate constant of 3.3±0.4×10 M · s at 23°C (8± 2×10 M · s at 37°C). Activation parameters for the reaction (ΔH‡, ΔS‡ and ΔV‡) revealed that the mechanism is rather associative than multi-step free-radical as expected for other thiols. This is in agreement with a primary formation of a new reaction product characterized by spectral and computational studies as HSNO (thionitrate), predominantly present as sulfinyl nitrite, HS(O)NO. This is the first time a thionitrate has been shown to be generated under biologically relevant conditions. The potential of HS(O)NO to serve as a NO donor in a pH-dependent manner and its ability to release NO inside the cells has been demonstrated. Thus sulfide modulates the chemistry and biological effects of peroxynitrite by its scavenging and formation of a new chemical entity (HSNO ) with the potential to release NO, suppressing the pro-apoptotic, oxidative and nitrative properties of peroxynitrite. Physiological concentrations of H S abrogated peroxynitrite-induced cell damage as demonstrated by the: (i) inhibition of apoptosis and necrosis caused by peroxynitrite; (ii) prevention of protein nitration; and (iii) inhibition of PARP-1 [poly(ADP-ribose) polymerase 1] activation in cellular models, implying that a major part of the cytoprotective effects of hydrogen sulfide may be mediated by modulation of peroxynitrite chemistry, in particular under inflammatory conditions. © The Authors Journal compilation © 2012 Biochemical Society.
APA:
Filipovic, M., Miljkovic, J., Allgäuer, A., Chaurio Gonzalez, R.A., Shubina, T., Herrmann, M., & Ivanovic-Burmazovic, I. (2012). Biochemical insight into physiological effects of H 2S: Reaction with peroxynitrite and formation of a new nitric oxide donor, sulfinyl nitrite. Biochemical Journal, 441(2), 609-621. https://doi.org/10.1042/BJ20111389
MLA:
Filipovic, Milos, et al. "Biochemical insight into physiological effects of H 2S: Reaction with peroxynitrite and formation of a new nitric oxide donor, sulfinyl nitrite." Biochemical Journal 441.2 (2012): 609-621.
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