A novel one-pot process for near-net-shape fabrication of open-porous resorbable hydroxyapatite/protein composites and in vivo assessment

Mueller B, Koch D, Lutz R, Schlegel KA, Treccani L, Rezwan K (2014)

Publication Type: Journal article

Publication year: 2014

Journal

Materials Science and Engineering C Elsevier

Book Volume: 42

Pages Range: 137-45

DOI: 10.1016/j.msec.2014.05.017

Abstract

We present a mild one-pot freeze gelation process for fabricating near-net, complex-shaped hydroxyapatite scaffolds and to directly incorporate active proteins during scaffold processing. In particular, the direct protein incorporation enables a simultaneous adjustment and control of scaffold microstructure, porosity, resorbability and enhancement of initial mechanical and handling stability. Two proteins, serum albumin and lysozyme, are selected and their effect on scaffold stability and microstructure investigated by biaxial strength tests, electron microscopy, and mercury intrusion porosimetry. The resulting hydroxyapatite/protein composites feature adjustable porosities from 50% to 70% and a mechanical strength ranging from 2 to 6 MPa comparable to that of human spongiosa without any sintering step. Scaffold degradation behaviour and protein release are assessed by in vitro studies. A preliminary in vivo assessment of scaffold biocompatibility and resorption behaviour in adult domestic pigs is discussed. After implantation, composites were resorbed up to 50% after only 4 weeks and up to 65% after 8 weeks. In addition, 14% new bone formation after 4 weeks and 37% after 8 weeks were detected. All these investigations demonstrate the outstanding suitability of the one-pot-process to create, in a customisable and reliable way, biocompatible scaffolds with sufficient mechanical strength for handling and surgical insertion, and for potential use as biodegradable bone substitutes and versatile platform for local drug delivery.

Authors with CRIS profile

Rainer Lutz Department of Oral and Cranio-Maxillofacial Surgery

Involved external institutions

Universität Bremen DE Germany (DE) Deutsches Zentrum für Luft- und Raumfahrt e.V. (DLR) / German Aerospace Center DE Germany (DE)

How to cite

APA:

Mueller, B., Koch, D., Lutz, R., Schlegel, K.A., Treccani, L., & Rezwan, K. (2014). A novel one-pot process for near-net-shape fabrication of open-porous resorbable hydroxyapatite/protein composites and in vivo assessment. Materials Science and Engineering C, 42, 137-45. https://doi.org/10.1016/j.msec.2014.05.017

MLA:

Mueller, Bent, et al. "A novel one-pot process for near-net-shape fabrication of open-porous resorbable hydroxyapatite/protein composites and in vivo assessment." Materials Science and Engineering C 42 (2014): 137-45.

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