Association of Kaposi's Sarcoma-Associated Herpesvirus ORF31 with ORF34 and ORF24 Is Critical for Late Gene Expression
Brulois K, Wong LY, Lee HR, Sivadas P, Enßer A, Feng P, Gao SJ, Toth Z, Jung JU (2015)
Publication Type: Journal article
Publication year: 2015
Journal
Book Volume: 89
Pages Range: 6148-54
Journal Issue: 11
DOI: 10.1128/JVI.00272-15
Abstract
Transcription of herpesvirus late genes depends on several virus-encoded proteins whose function is not completely understood. Here, we identify a viral trimeric complex of Kaposi's sarcoma-associated herpesvirus (KSHV) open reading frame 31 (ORF31), ORF24, and ORF34 that is required for late gene expression but not viral DNA replication. We found that (i) ORF34 bridges the interaction between ORF31 and ORF24, (ii) the amino-terminal cysteine-rich and carboxyl-terminal basic domains of ORF31 mediate the ORF31-ORF34 interaction required for late gene expression, and (iii) a complex consisting of ORF24, ORF31, and ORF34 specifically binds to the K8.1 late promoter. Together, our results support the model that a subset of lytic viral proteins assembles into a transcriptional activator complex to induce expression of late genes.
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APA:
Brulois, K., Wong, L.-Y., Lee, H.-R., Sivadas, P., Enßer, A., Feng, P.,... Jung, J.U. (2015). Association of Kaposi's Sarcoma-Associated Herpesvirus ORF31 with ORF34 and ORF24 Is Critical for Late Gene Expression. Journal of Virology, 89(11), 6148-54. https://dx.doi.org/10.1128/JVI.00272-15
MLA:
Brulois, Kevin, et al. "Association of Kaposi's Sarcoma-Associated Herpesvirus ORF31 with ORF34 and ORF24 Is Critical for Late Gene Expression." Journal of Virology 89.11 (2015): 6148-54.
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