Force-induced growth of adhesion domains is controlled by receptor mobility

Smith AS, Sengupta K, Goennenwein S, Seifert U, Sackmann E (2008)

Publication Language: English

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2008

Journal

Proceedings of the National Academy of Sciences of the United States of America National Academy of Sciences

Publisher: NATL ACAD SCIENCES

Book Volume: 105

Pages Range: 6906-6911

Journal Issue: 19

URI: http://www.pnas.org/content/105/19/6906.full.pdf

DOI: 10.1073/pnas.0801706105

Abstract

In living cells, adhesion structures have the astonishing ability to grow and strengthen under force. Despite the rising evidence of the importance of this phenomenon, little is known about the underlying mechanism. Here, we show that force-induced adhesion-strengthening can occur purely because of the thermodynamic response to the elastic deformation of the membrane, even in the absence of the actively regulated cytoskeleton of the cell, which was hitherto deemed necessary. We impose pN-forces on two fluid membranes, locally pre-adhered by RGD-integrin binding. One of the binding partners is always mobile whereas the mobility of the other can be switched on or off. immediate passive strengthening of adhesion structures occurs in both cases. When both binding partners are mobile, strengthening is aided by lateral movement of intact bonds as a transient response to force-induced membrane-deformation. By extending our microinterferometric technique to the suboptical regime, we show that the adhesion, as well as the resistance to force-induced de-adhesion, is greatly enhanced when both, rather than only one, of the binding partners are mobile. We formulate a theory that explains our observations by linking the macroscopic shape deformation with the microscopic formation of bonds, which further elucidates the importance of receptor mobility. We propose this fast passive response to be the first-recognition that triggers signaling events leading to mechanosensing in living cells.

Authors with CRIS profile

Ana-Suncana Smith Physics Underlying Life Science

Involved external institutions

Forschungszentrum Jülich / Research Centre Jülich (FZJ) DE Germany (DE) Technische Universität München (TUM) DE Germany (DE) Universität Stuttgart DE Germany (DE)

How to cite

APA:

Smith, A.-S., Sengupta, K., Goennenwein, S., Seifert, U., & Sackmann, E. (2008). Force-induced growth of adhesion domains is controlled by receptor mobility. Proceedings of the National Academy of Sciences of the United States of America, 105(19), 6906-6911. https://dx.doi.org/10.1073/pnas.0801706105

MLA:

Smith, Ana-Suncana, et al. "Force-induced growth of adhesion domains is controlled by receptor mobility." Proceedings of the National Academy of Sciences of the United States of America 105.19 (2008): 6906-6911.

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