Treatment Efficiency of Free and Nanoparticle-Loaded Mitoxantrone for Magnetic Drug Targeting in Multicellular Tumor Spheroids

Journal article


Publication Details

Author(s): Hornung A, Poettler M, Friedrich RP, Zaloga J, Unterweger H, Lyer S, Nowak J, Odenbach S, Alexiou C, Janko C
Journal: Molecules
Publication year: 2015
Volume: 20
Journal issue: 10
Pages range: 18016-30
ISSN: 1420-3049


Abstract


Major problems of cancer treatment using systemic chemotherapy are severe side effects. Magnetic drug targeting (MDT) employing superparamagnetic iron oxide nanoparticles (SPION) loaded with chemotherapeutic agents may overcome this dilemma by increasing drug accumulation in the tumor and reducing toxic side effects in the healthy tissue. For translation of nanomedicine from bench to bedside, nanoparticle-mediated effects have to be studied carefully. In this study, we compare the effect of SPION, unloaded or loaded with the cytotoxic drug mitoxantrone (MTO) with the effect of free MTO, on the viability and proliferation of HT-29 cells within three-dimensional multicellular tumor spheroids. Fluorescence microscopy and flow cytometry showed that both free MTO, as well as SPION-loaded MTO (SPION(MTO)) are able to penetrate into tumor spheroids and thereby kill tumor cells, whereas unloaded SPION did not affect cellular viability. Since SPION(MTO) has herewith proven its effectivity also in complex multicellular tumor structures with its surrounding microenvironment, we conclude that it is a promising candidate for further use in magnetic drug targeting in vivo.



FAU Authors / FAU Editors

Alexiou, Christoph Prof. Dr.
Professur für Nanomedizin
Janko, Christina Dr. rer. nat.
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie
Lyer, Stefan Dr. rer. nat.
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie
Unterweger, Harald Dr.-Ing.
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie
Zaloga, Jan Dr. rer. nat.
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie


Additional Organisation
Exzellenz-Cluster Engineering of Advanced Materials


External institutions
Technische Universität Dresden


How to cite

APA:
Hornung, A., Poettler, M., Friedrich, R.P., Zaloga, J., Unterweger, H., Lyer, S.,... Janko, C. (2015). Treatment Efficiency of Free and Nanoparticle-Loaded Mitoxantrone for Magnetic Drug Targeting in Multicellular Tumor Spheroids. Molecules, 20(10), 18016-30. https://dx.doi.org/10.3390/molecules201018016

MLA:
Hornung, Annkathrin, et al. "Treatment Efficiency of Free and Nanoparticle-Loaded Mitoxantrone for Magnetic Drug Targeting in Multicellular Tumor Spheroids." Molecules 20.10 (2015): 18016-30.

BibTeX: 

Last updated on 2018-09-10 at 02:22