Association Rates of Membrane-Coupled Cell Adhesion Molecules

Beitrag in einer Fachzeitschrift

Details zur Publikation

Autor(en): Bihr T, Fenz S, Sackmann E, Merkel R, Seifert U, Sengupta K, Smith AS
Zeitschrift: Biophysical Journal
Jahr der Veröffentlichung: 2014
Band: 107
Heftnummer: 11
Seitenbereich: L33-L36
ISSN: 0006-3495
Sprache: Englisch


Thus far, understanding how the confined cellular environment affects the lifetime of bonds, as well as the extraction of complexation rates, has been a major challenge in studies of cell adhesion. Based on a theoretical description of the growth curves of adhesion domains, we present a new (to our knowledge) method to measure the association rate k(on) of ligand-receptor pairs incorporated into lipid membranes. As a proof of principle, we apply this method to several systems. We find that the k(on) for the interaction of biotin with neutravidin is larger than that for integrin binding to RGD or sialyl Lewis(x) to E-selectin. Furthermore, we find k(on) to be enhanced by membrane fluctuations that increase the probability for encounters between the binders. The opposite effect on k(on) could be attributed to the presence of repulsive polymers that mimic the glycocalyx, which points to two potential mechanisms for controlling the speed of protein complexation during the cell recognition process.

FAU-Autoren / FAU-Herausgeber

Bihr, Timo
Professur für Theoretische Physik
Smith, Ana-Suncana Prof. Dr.
Professur für Theoretische Physik

Zusätzliche Organisationseinheit(en)
Exzellenz-Cluster Engineering of Advanced Materials

Autor(en) der externen Einrichtung(en)
Aix-Marseille University / Aix-Marseille Université
Forschungszentrum Jülich GmbH (FZJ)
Technische Universität München (TUM)
Universität Stuttgart


Bihr, T., Fenz, S., Sackmann, E., Merkel, R., Seifert, U., Sengupta, K., & Smith, A.-S. (2014). Association Rates of Membrane-Coupled Cell Adhesion Molecules. Biophysical Journal, 107(11), L33-L36.

Bihr, Timo, et al. "Association Rates of Membrane-Coupled Cell Adhesion Molecules." Biophysical Journal 107.11 (2014): L33-L36.


Zuletzt aktualisiert 2018-10-08 um 17:09