Association Rates of Membrane-Coupled Cell Adhesion Molecules

Bihr T, Fenz S, Sackmann E, Merkel R, Seifert U, Sengupta K, Smith AS (2014)


Publication Language: English

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2014

Journal

Publisher: CELL PRESS

Book Volume: 107

Pages Range: L33-L36

Journal Issue: 11

URI: http://www.sciencedirect.com/science/article/pii/S0006349514011163

DOI: 10.1016/j.bpj.2014.10.033

Abstract

Thus far, understanding how the confined cellular environment affects the lifetime of bonds, as well as the extraction of complexation rates, has been a major challenge in studies of cell adhesion. Based on a theoretical description of the growth curves of adhesion domains, we present a new (to our knowledge) method to measure the association rate k(on) of ligand-receptor pairs incorporated into lipid membranes. As a proof of principle, we apply this method to several systems. We find that the k(on) for the interaction of biotin with neutravidin is larger than that for integrin binding to RGD or sialyl Lewis(x) to E-selectin. Furthermore, we find k(on) to be enhanced by membrane fluctuations that increase the probability for encounters between the binders. The opposite effect on k(on) could be attributed to the presence of repulsive polymers that mimic the glycocalyx, which points to two potential mechanisms for controlling the speed of protein complexation during the cell recognition process.

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How to cite

APA:

Bihr, T., Fenz, S., Sackmann, E., Merkel, R., Seifert, U., Sengupta, K., & Smith, A.-S. (2014). Association Rates of Membrane-Coupled Cell Adhesion Molecules. Biophysical Journal, 107(11), L33-L36. https://dx.doi.org/10.1016/j.bpj.2014.10.033

MLA:

Bihr, Timo, et al. "Association Rates of Membrane-Coupled Cell Adhesion Molecules." Biophysical Journal 107.11 (2014): L33-L36.

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