Human dendritic cell subsets display distinct interactions with the pathogenic mould Aspergillus fumigatus

Journal article


Publication Details

Author(s): Lother J, Breitschopf T, Krappmann S, Morton CO, Bouzani M, Kurzai O, Gunzer M, Hasenberg M, Einsele H, Loeffler J
Journal: International Journal of Medical Microbiology
Publisher: Elsevier
Publication year: 2014
Volume: 304
Journal issue: 8
Pages range: 1160-8
ISSN: 1438-4221


Abstract


The mould Aspergillus fumigatus is primarily an opportunistic pathogen of immunocompromised patients. Once fungal spores have been inhaled they encounter cells of the innate immune system, which include dendritic cells (DCs). DCs are the key antigen-presenting cells of the immune system and distinct subtypes, which differ in terms of origin, morphology and function. This study has systematically compared the interactions between A. fumigatus and myeloid DCs (mDCs), plasmacytoid DCs (pDCs) and monocyte-derived DCs (moDCs). Analyses were performed by time-lapse video microscopy, scanning electron microscopy, plating assays, flow cytometry, 25-plex ELISA and transwell assays. The three subsets of DCs displayed distinct responses to the fungus with mDCs and moDCs showing the greatest similarities. mDCs and moDCs both produced rough convolutions and occasionally phagocytic cups upon exposure to A. fumigatus whereas pDCs maintained a smooth appearance. Both mDCs and moDCs phagocytosed conidia and germ tubes, while pDCs did not phagocytose any fungi. Analysis of cytokine release and maturation markers revealed specific differences in pro- and anti-inflammatory patterns between the different DC subsets. These distinct characteristics between the DC subsets highlight their differences and suggest specific roles of moDCs, mDCs and pDCs during their interaction with A. fumigatus in vivo.



FAU Authors / FAU Editors

Krappmann, Sven Prof. Dr.
Professur für Klinische Mikrobiologie und Immunologie


External institutions with authors

Friedrich-Schiller-Universität Jena
Universität Duisburg-Essen (UDE)
Universitätsklinikum Würzburg
University of Western Sydney


How to cite

APA:
Lother, J., Breitschopf, T., Krappmann, S., Morton, C.O., Bouzani, M., Kurzai, O.,... Loeffler, J. (2014). Human dendritic cell subsets display distinct interactions with the pathogenic mould Aspergillus fumigatus. International Journal of Medical Microbiology, 304(8), 1160-8. https://dx.doi.org/10.1016/j.ijmm.2014.08.009

MLA:
Lother, Jasmin, et al. "Human dendritic cell subsets display distinct interactions with the pathogenic mould Aspergillus fumigatus." International Journal of Medical Microbiology 304.8 (2014): 1160-8.

BibTeX: 

Last updated on 2018-09-10 at 02:20