KLF2-a negative regulator of pre-B cell clonal expansion and B cell activation

Journal article


Publication Details

Author(s): Winkelmann R, Sandrock L, Kirberg J, Jäck HM, Schuh W
Journal: PLoS ONE
Publication year: 2014
Volume: 9
Journal issue: 5
Pages range: e97953
ISSN: 1932-6203


Abstract


Maturation as well as antigen-dependent activation of B cells is accompanied by alternating phases of proliferation and quiescence. We and others have previously shown that Krüppel-like factor 2 (KLF2), a regulator of T cell quiescence and migration, is upregulated in small resting precursor (pre)-B cells after assembly of the immature pre-B cell receptor (pre-BCR) and is downregulated upon antigen-induced proliferation of mature B cells. These findings suggest that KLF2, besides its function in maintaining follicular B cell identity, peripheral B cell homeostasis and homing of antigen-specific plasma cells to the bone marrow, also controls clonal expansion phases in the B cell lineage. Here, we demonstrate that enforced expression of KLF2 in primary pre-B cells results in a severe block of pre-BCR-induced proliferation, upregulation of the cell cycle inhibitors p21 and p27 and downregulation of c-myc. Furthermore, retroviral KLF2 transduction of primary B cells impairs LPS-induced activation, favors apoptosis and results in reduced abundance of factors, such as AID, IRF4 and BLIMP1, that control the antigen-dependent phase of B cell activation and plasma cell differentiation. Hence, we conclude that KLF2 is not only a key player in terminating pre-B cell clonal expansion but also a potent suppressor of B cell activation.



FAU Authors / FAU Editors

Jäck, Hans-Martin Prof. Dr.
Molekular-Immunologische Abteilung in der Medizinischen Klinik 3
Schuh, Wolfgang Dr. rer. nat.
Molekular-Immunologische Abteilung in der Medizinischen Klinik 3
Winkelmann, Rebecca Dr.
Professur für Immunologie


External institutions with authors

Paul Ehrlich Institute / Paul-Ehrlich-Institut – Bundesinstitut für Impfstoffe und biomedizinische Arzneimittel (PEI)


How to cite

APA:
Winkelmann, R., Sandrock, L., Kirberg, J., Jäck, H.-M., & Schuh, W. (2014). KLF2-a negative regulator of pre-B cell clonal expansion and B cell activation. PLoS ONE, 9(5), e97953. https://dx.doi.org/10.1371/journal.pone.0097953

MLA:
Winkelmann, Rebecca, et al. "KLF2-a negative regulator of pre-B cell clonal expansion and B cell activation." PLoS ONE 9.5 (2014): e97953.

BibTeX: 

Last updated on 2018-09-10 at 02:18