Reaction of folic acid with reducing sugars and sugar degradation products

Journal article


Publication Details

Author(s): Schneider M, Klotzsche M, Werzinger C, Hegele J, Waibel R, Pischetsrieder M
Journal: Journal of Agricultural and Food Chemistry
Publisher: American Chemical Society
Publication year: 2002
Volume: 50
Pages range: 1647-1651
ISSN: 0021-8561


Abstract


The reaction of folic acid with reducing sugars (nonenzymatic glycation) under conditions that can occur during food processing and preparation was studied by high-performance liquid chromatography with diode array detection. N-(p-Aminobenzoyl)-L-glutamic acid, a well-established oxidation product, was detected in the reaction mixtures. Furthermore, a new product was isolated and identified as N2-[1-(carboxyethyl)]folic acid (CEF). CEF was the main product that was formed by the nonenzymatic glycation of folic acid. For preparation, N2-[1-(carboxyethyl)]folic acid was obtained in high yields when folic acid and dihydroxyacetone (DHA), a sugar degradation product, were heated at 100°C in phosphate buffer. Mixtures of folic acid and different sugars or DHA were heated under variation of reaction time and temperature, and CEF was quantified. Up to 50% of the vitamin was converted to CEF, with highest yields formed from maltose (49%) and lactose (43%).



FAU Authors / FAU Editors

Pischetsrieder, Monika Prof. Dr.
Lehrstuhl für Lebensmittelchemie (Henriette-Schmidt-Burkhardt Lehrstuhl)
Waibel, Reiner Dr.
Lehrstuhl für Pharmazeutische Chemie


Additional Organisation
Emil-Fischer-Zentrum (Emil Fischer Center)


How to cite

APA:
Schneider, M., Klotzsche, M., Werzinger, C., Hegele, J., Waibel, R., & Pischetsrieder, M. (2002). Reaction of folic acid with reducing sugars and sugar degradation products. Journal of Agricultural and Food Chemistry, 50, 1647-1651. https://dx.doi.org/10.1021/jf011042p

MLA:
Schneider, Marc, et al. "Reaction of folic acid with reducing sugars and sugar degradation products." Journal of Agricultural and Food Chemistry 50 (2002): 1647-1651.

BibTeX: 

Last updated on 2018-19-04 at 02:39