The endoplasmic reticulum is the main membrane source for biogenesis of the lytic vacuole in Arabidopsis

Viotti C, Krüger F, Krebs M, Neubert C, Fink F, Lupanga U, Scheuring D, Boutté Y, Frescatada-Rosa M, Wolfenstetter S, Sauer N, Hillmer S, Grebe M, Schumacher K (2013)

Publication Language: English

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2013

Journal

The Plant Cell American Society of Plant Biologists

Book Volume: 25

Pages Range: 3434-3449

Journal Issue: 9

DOI: 10.1105/tpc.113.114827

Open Access Link: http://www.plantcell.org/content/25/9/3434.long#xref-corresp-1-1

Abstract

Vacuoles are multifunctional organelles essential for the sessile lifestyle of plants. Despite their central functions in cell growth, storage, and detoxification, knowledge about mechanisms underlying their biogenesis and associated protein trafficking pathways remains limited. Here, we show that in meristematic cells of the Arabidopsis thaliana root, biogenesis of vacuoles as well as the trafficking of sterols and of two major tonoplast proteins, the vacuolar H+-pyrophosphatase and the vacuolar H+-adenosinetriphosphatase, occurs independently of endoplasmic reticulum (ER)-Golgi and post-Golgi trafficking. Instead, both pumps are found in provacuoles that structurally resemble autophagosomes but are not formed by the core autophagy machinery. Taken together, our results suggest that vacuole biogenesis and trafficking of tonoplast proteins and lipids can occur directly from the ER independent of Golgi function. © 2013 American Society of Plant Biologists. All rights reserved.

Authors with CRIS profile

Susanne Wolfenstetter Lehrstuhl für Molekulare Pflanzenphysiologie Norbert Sauer Lehrstuhl für Molekulare Pflanzenphysiologie

Involved external institutions

Ruprecht-Karls-Universität Heidelberg DE Germany (DE) Umeå University SE Sweden (SE)

How to cite

APA:

Viotti, C., Krüger, F., Krebs, M., Neubert, C., Fink, F., Lupanga, U.,... Schumacher, K. (2013). The endoplasmic reticulum is the main membrane source for biogenesis of the lytic vacuole in Arabidopsis. The Plant Cell, 25(9), 3434-3449. https://dx.doi.org/10.1105/tpc.113.114827

MLA:

Viotti, Corrado, et al. "The endoplasmic reticulum is the main membrane source for biogenesis of the lytic vacuole in Arabidopsis." The Plant Cell 25.9 (2013): 3434-3449.

BibTeX: Download