Comparison of Different Matrices as Potential Quality Control Samples for Neurochemical Dementia Diagnostics

Lelental N, Brandner S, Kofanova O, Blennow K, Zetterberg H, Andreasson U, Engelborghs S, Mroczko B, Gabryelewicz T, Teunissen C, Mollenhauer B, Parnetti L, Chiasserini D, Molinuevo JL, Perret-Liaudet A, Verbeek MM, Andreasen N, Brosseron F, Bahl JMC, Herukka SK, Hausner L, Froelich L, Labonte A, Poirier J, Miller AM, Zilka N, Kovacech B, Urbani A, Suardi S, Oliveira C, Baldeiras I, Dubois B, Rot U, Lehmann S, Skinningsrud A, Betsou F, Wiltfang J, Gkatzima O, Winblad B, Buchfelder M, Kornhuber J, Lewczuk P (2016)

Publication Type: Journal article

Publication year: 2016

Journal

Book Volume: 52

Pages Range: 51-64

Journal Issue: 1

DOI: 10.3233/JAD-150883

Abstract

Assay-vendor independent quality control (QC) samples for neurochemical dementia diagnostics (NDD) biomarkers are so far commercially unavailable. This requires that NDD laboratories prepare their own QC samples, for example by pooling leftover cerebrospinal fluid (CSF) samples.To prepare and test alternative matrices for QC samples that could facilitate intra- and inter-laboratory QC of the NDD biomarkers.Three matrices were validated in this study: (A) human pooled CSF, (B) A? peptides spiked into human prediluted plasma, and (C) A? peptides spiked into solution of bovine serum albumin in phosphate-buffered saline. All matrices were tested also after supplementation with an antibacterial agent (sodium azide). We analyzed short- and long-term stability of the biomarkers with ELISA and chemiluminescence (Fujirebio Europe, MSD, IBL International), and performed an inter-laboratory variability study.NDD biomarkers turned out to be stable in almost all samples stored at the tested conditions for up to 14 days as well as in samples stored deep-frozen (at - 80°C) for up to one year. Sodium azide did not influence biomarker stability. Inter-center variability of the samples sent at room temperature (pooled CSF, freeze-dried CSF, and four artificial matrices) was comparable to the results obtained on deep-frozen samples in other large-scale projects.Our results suggest that it is possible to replace self-made, CSF-based QC samples with large-scale volumes of QC materials prepared with artificial peptides and matrices. This would greatly facilitate intra- and inter-laboratory QC schedules for NDD measurements.

Authors with CRIS profile

Involved external institutions

Integrated Biobank of Luxembourg: IBBL Luxembourg (LU) Karolinska Institute Sweden (SE) Aristotle University of Thessaloniki Greece (GR) Georg-August-Universität Göttingen Germany (DE) Akershus University Hospital / Akershus universitetssykehus (Ahus) Norway (NO) Centre Hospitalier Universitaire de Montpellier (CHU/CHRU MTP) France (FR) Universitätsklinikum Heidelberg Germany (DE) University of Paris 6 - Pierre et Marie Curie / Université Paris VI Pierre et Marie Curie (UPMC) France (FR) Universidade de Coimbra Portugal (PT) Foundation of the Carlo Besta Neurological Institute (IRCCS) Italy (IT) Foundation Saint Lucia Italy (IT) Slovak Academy of Sciences (SAS) / Slovenská akadémia vied (SAV) Slovakia (SK) Trinity College Dublin Ireland (IE) Douglas Mental Health University Institute Canada (CA) Ruprecht-Karls-Universität Heidelberg Germany (DE) University of Eastern Finland Finland (FI) Statens Serum Institut Denmark (DK) Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE) Germany (DE) Karolinska University Hospital / Karolinska Universitetssjukhuset Sweden (SE) Vrije Universiteit Amsterdam (VU) / University Amsterdam Netherlands (NL) Université Claude Bernard Lyon 1 (UCB) France (FR) Radboud University Nijmegen Netherlands (NL) Hospital Clínic de Barcelona Spain (ES) Università degli Studi di Perugia Italy (IT) Paracelsus-Elena-Klinik Kassel Germany (DE) Polska Akademia Nauk (PAN) / Polish Academy of Sciences Poland (PL) University of Białystok Poland (PL) University of Antwerp / Universiteit Antwerpen Belgium (BE) University of Gothenburg / Göteborgs universitet Sweden (SE)

How to cite

APA:

Lelental, N., Brandner, S., Kofanova, O., Blennow, K., Zetterberg, H., Andreasson, U.,... Lewczuk, P. (2016). Comparison of Different Matrices as Potential Quality Control Samples for Neurochemical Dementia Diagnostics. Journal of Alzheimer's Disease, 52(1), 51-64. https://dx.doi.org/10.3233/JAD-150883

MLA:

Lelental, Natalia, et al. "Comparison of Different Matrices as Potential Quality Control Samples for Neurochemical Dementia Diagnostics." Journal of Alzheimer's Disease 52.1 (2016): 51-64.

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